Merck
  • Home
  • Search Results
  • Pulsed stable isotope labeling of amino acids in cell culture uncovers the dynamic interactions between HIV-1 and the monocyte-derived macrophage.

Pulsed stable isotope labeling of amino acids in cell culture uncovers the dynamic interactions between HIV-1 and the monocyte-derived macrophage.

Journal of proteome research (2011-04-20)
Stephanie D Kraft-Terry, Ian L Engebretsen, Dhundy K Bastola, Howard S Fox, Pawel Ciborowski, Howard E Gendelman
ABSTRACT

Dynamic interactions between human immunodeficiency virus-1 (HIV-1) and the macrophage govern the tempo of viral dissemination and replication in its human host. HIV-1 affects macrophage phenotype, and the macrophage, in turn, can modulate the viral life cycle. While these processes are linked to host-cell function and survival, the precise intracellular pathways involved are incompletely understood. To elucidate such dynamic virus-cell events, we employed pulsed stable isotope labeling of amino acids in cell culture. Alterations in de novo protein synthesis of HIV-1 infected human monocyte-derived macrophages (MDM) were examined after 3, 5, and 7 days of viral infection. Synthesis rates of cellular metabolic, regulatory, and DNA packaging activities were decreased, whereas, those affecting antigen presentation (major histocompatibility complex I and II) and interferon-induced antiviral activities were increased. Interestingly, enrichment of proteins linked to chromatin assembly or disassembly, DNA packaging, and nucleosome assembly were identified that paralleled virus-induced cytopathology and replication. We conclude that HIV-1 regulates a range of host MDM proteins that affect its survival and abilities to contain infection.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-Arginine-13C6,15N4 hydrochloride, 99 atom % 15N, 99 atom % 13C, 95% (CP)
Sigma-Aldrich
L-Lysine-13C6 hydrochloride, 99 atom % 13C, 95% (CP)
Sigma-Aldrich
L-Arginine-13C6 hydrochloride, 99 atom % 13C, 95% (CP)