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Optimization and Validation of an HPAEC-PAD Method for the Quantification of FODMAPs in Cereals and Cereal-Based Products.

Journal of agricultural and food chemistry (2019-03-28)
Lilit Ispiryan, Mareile Heitmann, Andrea Hoehnel, Emanuele Zannini, Elke K Arendt
ABSTRACT

This study presents an analytical method for the quantification of fermentable oligo-, di-, and monosaccharides and polyols (FODMAPs) in cereals and cereal-based products, considering diverse ingredients, such as different cereals in addition to wheat, pulses, or pseudocereals. All carbohydrates have been separated, identified, and quantified with a high-performance anion-exchange chromatographic system coupled with a pulsed amperometric detection (HPAEC-PAD). The total fructan content and the average degree of polymerization (DPav) have been determined after enzymatic hydrolysis to the monomers glucose and fructose, on the basis of the principle of the official method for fructan quantification in food products, AOAC 997.08. The methods for extraction, separation, and detection as well as fructan determination are based on several other studies and were modified in order to minimize interferences in the analysis. The method has been validated with regard to the limits of detection and quantification, the linearity, the repeatability, and the accuracy as well as the DPav of the fructans.

MATERIALS
Product Number
Brand
Product Description

Supelco
Nystose, analytical standard
Supelco
1-Kestose, analytical standard
Sigma-Aldrich
L-Rhamnose, natural sourced, 99%, FG