HomeSupportDetergent-based Western Blot Stripping Buffer

Detergent-based Western Blot Stripping Buffer

Stripping and re-probing a Western blot enables sample conservation and fast correction or adjustments of antibody concentration and incubation time. This recipe is for a Western blot stripping solution that uses the surfactant sodium dodecyl sulfate (SDS) and the reducing agent β-mercaptoethanol (BME) to remove antibodies and chemiluminescent or fluorescent detection reagents.

This calculator enables the accurate preparation of this detergent-based Western blot stripping solution for any milliliter volume. Input your desired volume, click the CALCULATE button, and the table will populate with the amounts of each component needed.

Detergent-based Western Blot Stripping Solution Recipe Calculator