Stripping and re-probing a Western blot enables sample conservation and fast correction or adjustments of antibody concentration and incubation time. This recipe is for an acidic Western blot stripping solution that uses glycine hydrochloride and the surfactant sodium dodecyl sulfate (SDS). If the target protein is sensitive to acidic conditions, try the detergent-based stripping solcution recipe.
This recipe calculator enables the accurate preparation of acidic Western blot stripping solution for any milliliter volume. Input your desired volume, click the CALCULATE button, and the table will populate with the amounts of each component needed.