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4X SDS Sample Loading Buffer for Western Blotting

This SDS sample loading buffer recipe is ideal for preparing and loading protein samples into gels for polyacrylamide gel electrophoresis analysis. This recipe calculator enables the accurate preparation of a 4X SDS sample loading buffer for any volume that you need. Input your desired volume, click the CALCULATE button, and the table will populate with the amounts of each component needed.

4X Solution concentrations:

  • Tris-HCl: 0.2 M
  • DTT: 0.4 M
  • SDS: 277 mM, 8.0% (w/v)
  • Bromophenol blue: 6 mM
  • Glycerol: 4.3 M