HomeSupport1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST) for Western Blotting

1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST) for Western Blotting

Tris-buffered saline with 0.1% Tween® 20 detergent (TBST) is an effective wash buffer for many immunoassays. To make 1 L of TBST wash buffer, add 100 mL of 10X TBS and 1 mL Tween® 20 detergent to 900 mL of water.

This calculator enables the accurate preparation of a 1X TBST working solution whether you are making enough for a single experiment or for the entire lab. Input your desired volume, click the CALCULATE button, and the table will populate with the amount of each component needed.

1X Solution concentrations:

  • Tris: 20 mM
  • NaCl: 150 mM
  • Tween® 20 detergent: 0.1% (w/v)

TBST Recipe Calculator