Tris-buffered saline with 0.1% Tween® 20 detergent (TBST) is an effective wash buffer for many immunoassays. To make 1 L of TBST wash buffer, add 100 mL of 10X TBS and 1 mL Tween® 20 detergent to 900 mL of water.
This calculator enables the accurate preparation of a 1X TBST working solution whether you are making enough for a single experiment or for the entire lab. Input your desired volume, click the CALCULATE button, and the table will populate with the amount of each component needed.
1X Solution concentrations: