Tris-buffered saline (TBS) is an excellent wash buffer for many types immunoassays, such as ELISAs, immunohistochemisty, and Western blots. To make 1 L of TBS wash buffer, add 100 mL of 10X TBS to 900 mL of water.
This calculator enables the accurate preparation of a 1X TBS working solution whether you are making enough for a single experiment or for the entire lab. Input your desired volume, click the CALCULATE button, and the table will populate with the amount of each component needed.
1X Solution concentrations: