HomeSupport1X Tris-Buffered Saline (TBS) Working Solution for Western Blotting

1X Tris-Buffered Saline (TBS) Working Solution for Western Blotting

Tris-buffered saline (TBS) is an excellent wash buffer for many types immunoassays, such as ELISAs, immunohistochemisty, and Western blots. To make 1 L of TBS wash buffer, add 100 mL of 10X TBS to 900 mL of water.

This calculator enables the accurate preparation of a 1X TBS working solution whether you are making enough for a single experiment or for the entire lab. Input your desired volume, click the CALCULATE button, and the table will populate with the amount of each component needed.

1X Solution concentrations:

  • Tris: 20 mM
  • NaCl: 150 mM

TBS Recipe Calculator