HomeSupport10X Phosphate-Buffered Saline (PBS) for Western Blotting

10X Phosphate-Buffered Saline (PBS) for Western Blotting

Phosphate-buffered saline (PBS) is an isotonic solution that is used in many biological research applications. This 10X PBS recipe contains 1.37 M NaCl, 27 mM KCl, 100 mM Na2HPO4, and 18 mM KH2PO4. To make 1 L of 10X PBS stock solution, combine 17.8 g of Na2HPO4, 2.4 g of KH2PO4, 80 g of NaCl, 2 g of KCl, and adjust final volume to 1 L.

This recipe calculator enables the accurate preparation of 10X PBS for any millimeter volume. Input your desired volume, click the CALCULATE button, and the table will populate with the amounts of each component needed.

10X Solution concentrations:

  • NaCl: 1.37 M
  • KCl: 27 mM
  • Na2HPO4: 100 mM
  • KH2PO4: 18 mM