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HPLC Analysis of Various Cationic Polymers on TSKgel® PWXL-CP Columns
Separation of Polyacrylic acid (PAA) 438 kDa; Polyacrylic acid (PAA) 235 kDa; Polyethylenimine (PEI) 266 kDa ; Poly(dimethyl diallyl ammonium chloride) (PIDADMACl) 204 kDa; p-Aminosalicylic acid (PAS) 7800 Da; p-Aminosalicylic acid (PAS) 257 kDa; Cationic dextran (11 kDa); Chitosan...
Nuclear Protein Extraction Without the Use of Detergent
A protocol for the preparation of nuclear proteins without the use of a detergent. Detergents can interfer with the labeling efficiency of the extracted proteins.
GC Analysis of Omega 3 Fatty Acids in Fish Oil Capsules and Farm Raised Salmon
nalysis of the omega 3 fatty acid profiles from fish oil capsules and salmon, highlighted through the use of 2 GC columns of different selectivity for analysts who wish to perform confirmatory analysis.
Rat Aortic Endothelial Cells (RAOEC) Culture Protocol
Technical information for working with rabbit aortic endothelial cells including thawing, subculturing and cryopreservation
Peptide Resin Loading Protocols
Protocols for loading various peptide resins
Determination of Water Content in Sunflower Oil Using Karl Fischer Titration
Summary application report for analysis of moisture in Sunflower oil.
Aminoglycoside Analysis in Pork Muscle using Molecularly Imprinted Polymer Cleanup and LC/MS/MS Detection
Extraction and quantitative analysis of aminoglycosides in porcine tissue, using molecular imprinted polymer solid phase extraction followed by LC-MS/MS.
REDExtract-N-Amp™ Seed PCR Kit Protocol
The REDExtract-N-Amp Seed PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from seeds (soybean, corn, wheat, etc.).
Sildenafil Citrate USP Method Using HPTLC Plates for the Limit of Imidazole Test
In the present study we demonstrate compliance of silica gel 60 F254 HPTLC plates with USP requirements, and how HPTLC plates are suitable for the Sildenafil citrate-Limit of Imidazole test.
Cancer Research Techniques
By focusing on analysis techniques and protocols specific for Cancer Research, Sigma-Aldrich is putting your research needs first. Protein Tyrosine Kinase and Phosphatase Expression Profiling in Cancer Research.
Mass Spectrometry Metabolite Library Protocol
MSMLS™ (Mass Spectrometry Metabolite Library of Standards) is a collection of high quality small biochemical molecules that span a broad range of primary metabolism. These are high purity (>95%) compounds supplied in an economical, ready-to-use format.
Coronavirus (SARS-CoV-2) Viral Proteins
Structural proteins of the SARS-CoV-2 virus are envelope (E), membrane (M), nucleocapsid (N), and spike (S). Find antibodies, inhibitors, and enzyme reagents for investigating coronavirus protein function.
Sepharose High Performance: Purification with High Resolution
This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.
Purification using StrepTrap™ HP 1 mL and 5 mL
This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from GE Healthcare.
Millicup™-FLEX Disposable Vacuum Filtration Units and demo video
Millicup™-FLEX disposable vacuum filter was designed to reduce contamination and save time. The solvent-resistant unit allows for filtration into storage bottles.
Protein Determination Modified Lowry Method
To standardize a procedure for the determination of protein by modified Lowry.
Coronavirus (SARS-CoV-2) Viral Proteins
Structural proteins of the SARS-CoV-2 virus are envelope (E), membrane (M), nucleocapsid (N), and spike (S). Find antibodies, inhibitors, and enzyme reagents for investigating coronavirus protein function.
Proteomic analysis of membrane proteins
This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from GE Healthcare.
Characteristics of Glutathione Sepharose® Products
Characteristics of Glutathione Sepharose® Products
Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol
The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format
Refolding using ion exchange chromatography and analysis of refolding
This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.
Duolink® PLA Probemaker Guide
Guide to create custom PLA probes for immunofluorescent or brightfield detection
Analytical Method: Analytical Quality Assurance, Standard for Hydrogen peroxide
Preparation of a standard solution for Hydrogen peroxide
SOURCE™: Purification at High Throughput with High Resolution and Easy Scale-Up
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
Sepharose Big Beads: Purification from Crude, Viscous Samples at Large Scale
This page covers how and why to use Sepharose Big Beads for purification from crude, viscous samples.
Protease Inhibition Using Protease Inhibitor Mix
Protease Inhibitor Mix from GE Healthcare inhibits serine, cysteine, metalloproteases, and calpain proteases and is suitable for animal and plant tissues, yeast, and bacteria.
Processing Small Tissue or Cell Samples using Sample Grinding Kit
Sample Grinding Kit from GE Healthcare is designed to disrupt cell or tissue samples for protein extraction.
Enzymatic Assay of Phosphoglucomutase (EC 5.4.2.2)
This procedure may be used for all Phosphoglucomutase products except for Phosphoglucomutase, Catalog Number P4109.
Counterstaining after the Duolink In Situ Protocol
We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.