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ChIC and CUT&RUN Data Analysis Tool Frequently Asked Questions (FAQs)
Chromatin Immunocleavage (ChIC) and CUT&RUN data analysis tool frequently asked questions (FAQs).
RNA Immunoprecipitation (RIP) FAQs
RNA Immunoprecipitation (RIP) Frequently Asked Questions
ChIP Troubleshooting
Frequently ask questions about ChIP.
Tips for Optimizing Immunofluorescence Protocols to Get the Best Image
Uses for immunofluorescence (IF)—where an antibody conjugated to a molecule that fluoresces when excited by lasers— include protein localization, confirmation of post-translational modification or activation, and proximity to/complexing with other proteins.
Duolink® PLA Troubleshooting Guide
Support information including tips and tricks, frequently asked questions, and basic troubleshooting.
Duolink® References
Find Duolink references based on the type of method used, post translational modification detected, and research focus.
Primary Antibodies for use with Duolink® PLA
Search for PLA validated primary antibodies and use with Duolink® PLA to amplify the signal 1000x. You can visually detect endogenous proteins in native cells.
Duolink® PLA Technology for Neuroscience Applications
Duolink proximity ligation assay used to study neuron interactions furthering neuroscience research.
How Proximity Ligation Assays (PLA) Work
Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.
Duolink® PLA Flow Cytometry Kits
Traditional flow cytometry has been limited in the ability to detect protein-protein interactions and low abundant proteins events — until now. We have combined Duolink® Proximity Ligation Assay (PLA) with flow cytometry in a convenient kit, making the analysis of...
How to Optimize Duolink® PLA for Flow Cytometry Detection
Considerations for proper experimental design, preparation and execution of the Duolink® PLA for flow cytometry protocol.
ChIP-X: Cross-linking proteins to DNA and cell lysis
X-ChIP: Cross-linking proteins to DNA and cell lysis
PEPscreen® Peptide Library Frequently Asked Questions (FAQ)
PEPscreen peptides are synthesized on proprietary, state-of-the-art robotic platforms utilizing optimized protocols based on Fmoc-chemistry.
ChIP Troubleshooting Tips
Chromatin Immunoprecipitation (ChIP) Troubleshooting Tips
Troubleshooting Guide for Duolink® PLA for Flow Cytometry Detection
General tips and tricks for proper experiment execution, aid in identifying potential problems, and provide solutions to ensure a successful Duolink® PLA experiment for flow cytometry.
Studies of Dimerization and Activation of Receptors in the EGFR-Family Using Duolink® Assays
Our aim was to investigate the dimerization and activation pattern of two receptors, EGFR (ErbB1) and HER2 (ErbB2), in the EGFR-family using breast cancer cell lines and selected colon tissues as model systems.
ChIC and CUT&RUN Data Analysis Tool Overview
Chromatin Immunocleavage (ChIC) and CUT&RUN data analysis tool for file management and downstream sequencing data analyses.
Duolink® PLA – Protein Detection Technology
Duolink based on Proximity Ligation Assay (in situ PLA) technology when combined with qualified antibodies outperforms traditional immunoassays, such as co-IP, through a simple and sensitive in situ detection of endogenous proteins. This technology enables you to visualize protein interactions...
RNA Immunoprecipitation Chip (RIP) Assay
RNA Immunoprecipitation (RIP) is an essential method for analyzing proteins that interact with and modify the function of mRNAs, small RNAs, viral RNAs, or lncRNAs.
Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection
Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.
Fragmentation of chromatin in ChIP
Fragmentation of chromatin in Chromatin Immunoprecipitation (ChIP)
Use of PEPscreen® Peptides to Investigate Protein Farnesyltransferase Substrate Specificity
Prenylation is an essential post-translational modification for the proper localization and function of many proteins and is catalyzed by protein farnesyltransferase (FTase) and protein geranylgeranyltransferase type I (GGTase-I). To identify potential novel prenylated proteins, we used a PEPscreen peptide library...
ChIP: Immunoprecipitation, washing and elution
Chromatin Immunoprecipitation (ChIP), washing and elution
Duolink® Proximity Ligation Assay Instructional Video
This video will show you step-by-step how to perform a Duolink® PLA experiment with cells on a slide that have already been fixed and permeabilized by the user.
ChIC/CUT&RUN Kits
Chromatin Immunocleavage (ChIC) kits and CUT&RUN technology overview for improved chromatin isolation and downstream analyses.
How to Optimize the Duolink® Proximity Ligation Assay
Things to consider for preparation, setup and execution of the Duolink® assay protocol
FLAG® HA System
The FLAG® HA tandem epitope system consists of small epitopes tags that are not eukaryotic derived. These features minimize interference with protein functions and provide superior specificity.
Sulfoxide-Containing Mass Spectrometry (MS)-Cleavable Cross-Linkers
Learn about homobifunctional sulfoxide-containing MS-cleavable cross-linkers that enables the capture of protein-protein interactions (PPIs) in native cellular environments, and the identification of cross-linked peptides permits the determination of both identity and connectivity of PPIs.
Guides to cell number for ChIP and endpoint analysis
Guides to cell number for Chromatin Immunoprecipitation (ChIP) and endpoint analysis
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