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Showing 1-13 of 13 results
Duolink® PLA Probemaker Guide
Guide to create custom PLA probes for immunofluorescent or brightfield detection
Counterstaining after the Duolink In Situ Protocol
We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.
RNA Immunoprecipitation qPCR (RIP- qPCR) Protocol
RNA Immunoprecipitation qPCR (RIP- qPCR) brief protocol
How to Create PLA®Probes
Duolink® kits use in situ PLA®, a proximity ligation assay technology, to accurately and objectively quantify individual proteins, and their interactions and modifications in unmodified cells and tissue.
Using Duolink® in protein interaction studies
The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.
Chromatin Isolation by RNA Purification (ChIRP) Protocol
Protocol for Chromatin Isolation by RNA Purification (ChIRP) Protocol
Duolink® PLA Flow Cytometry Protocol
Protocol for use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry.
Purification or Removal of DNA-Binding Proteins
This page shows how to purify or remove DNA-binding proteins with Heparin Sepharose High Performance, Heparin Sepharose 6 Fast Flow, Capto Heparin from GE Healthcare.
Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
Using Duolink™ in Multiwell Plates
When using the Duolink reagents to stain cells grown in multiwell plates, a few modifications to the Duolink protocol, optimized for staining samples deposited on glass slides, have to be made in order to suit the multiwell format. In particular...
Duolink® PLA Multicolor Detection Protocol
Duolink® PLA Multicolor Detection Protocol