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Purification of Thiol-Containing Substances By Covalent Chromatography
Thiol-containing substances can be isolated selectively by covalent binding to an activated thiolated matrix via thiol-disulphide exchange to form a mixed disulphide bond. After washing away unbound material, the thiol-containing substance is eluted by reducing the disulphide bond. This technique...
Immobilized Metal Chelate Affinity Chromatography (IMAC)
This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from GE Healthcare.
Ion Exchange Chromatography
Ion exchange chromatography is employed by the Genopure Plasmid Midi Kit and the Genopure Plasmid Maxi Kit.
Lewatit® Ion Exchange Resins – All-around Solutions for Chemical Processes
Chemical processes comprise the steps of processing chemical starting materials, synthesizing the products, isolating the generated materials from the reaction mixture and subsequent purification.
Viral Clearance Using Capto™ Adhere
Description of viral clearance using Capto adhere from GE Healthcare.
Troubleshooting Reversed Phase Chromatography (RPC)
Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.
Troubleshooting Guide for Affinity Chromatography of Tagged Proteins
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
Desalting and Buffer Exchange for Affinity Chromatography of Antibodies
This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.
Principles and Standard Conditions for Different Purification Techniques
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.
Capto Adhere
Capto adhere from GE Healthcare is a multimodal strong anion exchanger for BioProcess applications.
Polishing of MAbs Using Capto Adhere ImpRes in Bind/Elute Mode
In these studies, the binding capacity for MAbs and the efficiency in the clearance of impurities using Capto adhere ImpRes in bind/elute mode was evaluated.
Purification or Removal of Viruses including Adeno-associated Virus
This page shows how to purify or remove viruses with a Capto DeVirS, AVB Sepharose High Performance from GE Healthcare.
LRA (Lipid Removal Agent) for Biopharmaceutical Production
Lipids are naturally-occurring hydrocarbons that are poorly soluble in water and soluble in non-polar solvents such as ether and chloroform.
Bind/Elute vs Flowthrough Mode in Multimodal Chromatography
This page describes the difference between bind/elute and flowthrough modes in multimodal chromatography using GE Healthcare products.
Setting Column Pressure Limits for Size Exclusion Chromatography
Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
Selection of Purification Equipment
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
Non-volatile and Volatile Buffer Systems
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
Comparison of Spherical and Irregular Silicas in Flash Chromatography
Comparison of Spherical and Irregular Silicas in Flash Chromatography
Optimization of Loading Conditions on Capto Adhere Using DoE
This page describes the optimization of loading conditions on Capto adhere from GE Healthcare using full factorial design of experiments (DoE).
Capto MMC
Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.
Purification Using Protein A-based Chromatography Media
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography
Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with GE Healthcare media are detailed, including sample application, load volume and temperature
Affinity Chromatography Troubleshooting
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Performing a purity and homogeneity check
This page shows how to perform a purification and homogeneity check of membrane proteins with products from GE Healthcare.
Maintenance of Multimodal Chromatography Media and Storage Conditions
This page describes the maintenance of media and storage conditions for multimodal chromatography using GE Healthcare products.
Protein G and protein A bind to different IgG
This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.