While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent
AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a speciﬁc ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, puriﬁcation
This page shows how to perform a separation with a Sephadex LH-20 column from GE Healthcare which are Size Exclusion Chromatography media of polysaccharide network made from cross-linked hydroxypropylated dextran beads.
This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.