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Sepharose High Performance: Purification with High Resolution
This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.
Purification using StrepTrap™ HP 1 mL and 5 mL
This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from GE Healthcare.
Characteristics of Glutathione Sepharose® Products
Characteristics of Glutathione Sepharose® Products
Refolding using ion exchange chromatography and analysis of refolding
This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.
Sepharose Big Beads: Purification from Crude, Viscous Samples at Large Scale
This page covers how and why to use Sepharose Big Beads for purification from crude, viscous samples.
Purification using MBPTrap™ HP Columns
This page shows how to purify MBP-tagged proteins using MBPTrap HP columns from GE Healthcare.
Reverse Phased Chromatography (RPC) in Practice
In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.
Removal of Detergents from Biological Product Matrices
The Amberlite™ XAD-4 resin used in Porozorb™ cartridges is a proven technology that is highly effective in removing various detergents from cell culture media for biopharmaceutical applications such as vaccine production.
Removal of GST Tag by Enzymatic Cleavage
This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.
Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up
This page covers the use of Sepharose Fast Flow for purification of proteins.
Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose High Performance from GE Healthcare.
Purification Using GSTrap™ HP, GSTrap™ FF, and GSTrap™ 4B columns
This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.
Purification of Histidine-Tagged Proteins using TALON® Superflow
This page shows how to purify histidine-tagged proteins using TALON Superflow from GE Healthcare.
Performing a Purification with Calmodulin Sepharose 4B
This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from GE Healthcare.
Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.
Capto Adhere ImpRes
Capto adhere ImpRes is a cost-effective and flexible chromatography medium from GE Healthcare, designed for high-resolution polishing of MAbs.
Capto Core 700
Capto Core 700 from GE Healthcare is designed for intermediate purification and polishing of viruses and other large biomolecules in flowthrough mode.
Packing a Column and Performing a Separation with Sephadex® LH-20
This page shows how to perform a separation with a Sephadex LH-20 column from GE Healthcare which are Size Exclusion Chromatography media of polysaccharide network made from cross-linked hydroxypropylated dextran beads.
Performing a Separation with Superose
Superose from GE Healthcare are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.
Tag Removal by Enzymatic Cleavage
This page how to remove histidine tags by enzymatic cleavage using GE Healthcare products.
Removal of Albumin Using Blue Sepharose® Chromatography Media
This page shows how to remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from GE Healthcare.
Coupling through the Primary Amine of a Ligand
This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.
Purification using StrepTactin Sepharose® High Performance
This page shows how to purify Strep-tag II recombinant proteins using StrepTactin Sepharose High Performance from GE Healthcare.
Sample Preparation in Ion Exchange Chromatography
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.
Selectivity and the Properties of a HIC Medium
While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent...
Column Packing and Preparation for Size Exclusion Chromatography
Column Packing and Preparation for Size Exclusion Chromatography
Refolding of proteins using gel filtration
This page shows how to refold proteins using gel filtration and products from GE Healthcare.
MonoBeads: Purification of Milligram Quantities with Highest Resolution
This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.