Applications
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Showing 1-30 of 76 results
HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX-NP5: Affect of pH
Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis;...
LC/MS Analysis of Amphetamine Enantiomers on Astec CHIROBIOTIC® V2 in Urine after Solid Phase Extraction (SPE) using Supel™-Select SCX
Shown here is the chiral separation of amphetamine enantiomers under MS-compatible conditions on Astec CHIROBIOTIC® V2 after extraction from urine using Supel™-Select SCX SPE 96-well plates. The highest grade mobile phase solvents and additives were used to supply low background...
Ascentis® Express RP-Amide HPLC Columns
Sigma-Aldrich.com presents an article about innovative particle technology and RP-Amide bonding chemistry provide the benefits of speed, resolution, selectivity, aqueous stability, and improved peak shape for bases.
HPLC Columns for Antibody and Large Protein Analysis
BIOshell™ IgG 1000 Å C4 columns are highly suited for the reversed phase separation of high molecular weight compounds such as monoclonal antibodies with molecular weight of 150 kDa.
UHPLC/MS Analysis of Diazepam and Metabolites on Titan™ C18, Comparison of Flow Rate and Column Length
Separation of Oxazepam; Desmethyldiazepam, analytical standard; Temazepam, analytical standard; Diazepam
Ascentis® Express OH5 U/HPLC Columns
High-speed Ascentis® Express OH5 HPLC columns are designed for enhanced HILIC partitioning and limited ion exchange retention
Milli-Q® Advantage A10 for Water Purification
It's intelligent. It's intuitive. It's designed to make your work as pleasant and comfortable as possible — and to maximize lab productivity. Its breakthrough ergonomic design and powerful purification media ensure consistent production of superior-quality ultrapure water. See how the...
HPLC Analysis of Xanthines on Purospher® STAR RP-18 endcapped
Xanthine is a purine base found in most human body tissues and fluids as well as in other organisms. Methylated xanthines (methylxanthines), which include caffeine, paraxanthine, theobromine, and theophylline, commonly used for their effects as mild stiµlants and as bronchodilators...
2 Micron SEC Column with Dual Functionality for Use in UHPLC and HPLC Systems
These columns are designed to be operated with a simple and well established method (sodium phosphate mobile phase, pH 6.8) and are packed for use in both HPLC and UHPLC systems.
Monolithic Silica with Bimodal Pore Structure
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
Ascentis® Express C18 U/HPLC Columns
Ascentis® Express C18 is the first choice when starting a new separation method.
Chromolith® HPLC and UHPLC columns
Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology.
How to Extend Liquid Chromatography Column Life: Regenerate or Use Guard Columns
What is causing aging of a column? Is the regeneration/washing of the column a reasonable approach to extend the lifetime of a column? What are the risks & challenges? Learn more in this article.
UHPLC Analysis of Conjugated Estrogens on Ascentis ® Express C18
Separation of Estriol 3-(β-D-glucuronide) sodium salt; β-Estradiol 3-(β-D-glucuronide) 17-sulfate dipotassium salt; Estriol 3-sulfate sodium salt; β-Estradiol 3,17-disulfate dipotassium salt, ≥95%; β-Estradiol 17-(β-D-glucuronide) sodium salt; β-Estradiol 3-(β-D-glucuronide) sodium salt; Estrone 3-(β-D-glucuronide) sodium salt; β-Estradiol 3-sulfate sodium salt, ≥93%; Estriol, ≥97%; Estrone...
Ascentis® Express RP-Amide Columns for HPLC and UHPLC
Improve your separation of basic compounds with our Ascentis® Express RP-Amide HPLC columns from the Supelco® portfolio.
Determination of Sucralose in Soy Sauce using HPLC-ELSD following the Chinese National Standard (GB) Method
The most frequently used synthetic sweeteners are: saccharin, cyclamate, aspartame, and sucralose (E955), and this application illustrate the analysis of sucralose from soy sauce following the current Chinese national standard method.
Fast Analysis of Monoclonal Antibodies Using Silica Monoliths Designed for Bioanalysis
Silica monoliths with larger mesopores are a powerful tool for the fast separation of biomolecules including mAbs. The immobilization of protein A on epoxy-modified widepore (WP) silica rods enabled highly reproducible and fast, one minute, separations of mAbs. The Chromolith®...
Ascentis® Express ES-Cyano Reversed Phase U/HPLC Columns
Ascentis® Express ES-Cyano HPLC column is a high-speed, high-performance liquid chromatography column based on 90 Å Fused-Core® particles.
Optimising the LC-MS Analysis of Biomolecules
Sigma-Aldrich.com presents an article concerning optimising the LC-MS analysis of biomolecules.
LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5
This application shows an Ascentis Express F5 provided rapid resolution of the isobaric vitamin D metabolites. MS detection provides the necessary secondary resolution.
Analytical Size-exclusion Chromatography Columns for Biomolecule Separations
Informative article on size exclusion chromatography columns for biomolecule separations. Using columns packed with sub-2 μm particles provides significant gains in efficiency over SEC columns packed with larger particles.
Chromatography Profile Analysis of Phosphorothioate Oligonucleotides
The ultimate goal of most antisense research is to develop therapeutic agents for diseases such as diabetes, cancer, and HIV/ AIDS. Phosphorothioate oligonucleotides (commonly referred to as S-oligos) are often the molecules of choice because of their resistance to cellular...
A quantitative mass spectrometry-based approach for identifying protein kinase clients and quantifying kinase activity
A quantitative mass spectrometry-based approach for identifying protein kinase clients and quantifying kinase activity
BIOshell™ Glycan U/HPLC Columns
The BIOshell™ Glycan column is ideal for the separation and quantitation of isolated glycans using the HILIC method for accurate glycoprotein analysis.
LC/MS/MS Analysis of Testosterone and Metabolites on Ascentis® Express C18
Separation of Testosterone solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material; 17α-Methyltestosterone solution, 1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material; 5α-Dihydrotestosterone (DHT) solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified...
Large Molecule BioAnalysis
Bioanalysis for large molecules, like Proteins or specific Biotherapeutics, refer to a set of methods, assays, and procedures that enable scientists to analyze specific proteins found in living organisms and the biochemical reactions underlying life processes.
Supelco Preparative HPLC products for Pharmaceutical Development and Production
Supelco Preparative HPLC products for Pharmaceutical Development and Production
Rapid Trypsin Digestion of Complex Protein Mixtures for Proteomics Analysis
Reliable and reproducible results in mass spectrometry proteomics analyses are highly dependent on robust sample preparation.