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Enzymatic Method for Determining Branched Chain Amino Acids (BCAA Assay)
Assay protocol for the colorimetric detection of Branched Chain Amino Acids (BCAA) in biological samples using the alanine assay kit.
β Secretase (BACE1) Activity Assay for BACE1 Inhibitor Screening
In recent years, the biological processes involved in the pathogenesis of Alzheimer’s disease (AD) have become increasingly better understood. It is now recognized that a key role is played by BACE1 proteases that cleave amyloid precursor protein (APP) to produce...
Excitatory Amino Acid Transporters
Sigma-Aldrich offers many products related to excitatory amino acid transporters for your research needs.
G Proteins (Heterotrimeric)
Sigma-Aldrich offers many products related to heterotrimeric G-proteins for your research needs.
Phospholipase D
Phosphatidylcholine (PC)-specific phospholipase D (PLD) enzymes catalyze the hydrolysis of PC to generate choline and phosphatidic acid. These enzymes belong to a superfamily of phosphatidyltransferases with a characteristic unique duplicated catalytic domain motif, the PLD or "€œHKD"€ domain flanked by...
Nuclear Receptors (PPARs)
Peroxisome proliferator activated receptors (PPARs) are members of the nuclear hormone receptor superfamily of ligand-activated transcription factors that are related to retinoid, steroid and thyroid hormone receptors.
Enzymatic Assay of Proteinase K with Hemoglobin Substrate
Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol...
PKA & PKG
Our offers many products related to PKA & PKG for your research needs.
Neuropeptidases
Sigma-Aldrich offers many products related to neuropeptidases for your research needs.
Bioluminescent Firefly Luciferase Assays
Firefly luciferase is a widely used bioluminescent reporter for studying gene regulation and function. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.
Enzyme Commission Numbers
Complete List of Enzyme Commission Numbers for metabolics research.
HMG-CoA Reductase (HMGR) Assay Kit
HMG-CoA Reductase (HMGR) Assay Kit
Phosphodiesterases
Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and/or cGMP. There are 11 different mammalian PDE families.
PKC
Sigma-Aldrich offers many products related to PKC for your research needs.
Enzymatic Assay of Chitosanase from Streptomyces Species
Enzymatic Assay of Chitosanase from Streptomyces Species
InsR
We offers many products related to InsR for your research needs.
How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Enzymatic Method for Determining Amylase Activity (Amylase Activity Assay)
Assay protocol for the colorimetricdetection of amylase activity in biological samples using the Amylase Activity assay kit.
Phospholipase C
We offers many products related to phospholipase C for your research needs.
Cholesterol Esterification
Cholesterol undergoes esterification to improve transport. Cholesterol esters are more easily packaged into the interior of lipoproteins - increasing the quantity that can be readily transported in the blood stream.
Enzymatic Assay of Deoxyribonuclease I (EC 3.1.21.1)
To standardize a procedure for the enzymatic assay of Deoxyribonuclease I at Sigma-Aldrich St. Louis.