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Applications:Cloning and Expression
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Showing 1-28 of 28 results
Universal Transfection Reagent Protocol
Sigma's Universal Transfection Reagent is a unique formulation of a proprietary polymer blend used for transient and stable transfection of nucleic acids into various eukaryotic cell lines and hard-to-transfect primary cells. Fast and easy protocol is compatible with serum, serum-free...
Competent Cell Protocols
Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.
X-tremeGENE HP DNA Transfection Reagent Protocol
Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Dephosphorylation Procedure for DNA and Proteins
Procedures for dephosphorylation featured at Sigma-Aldrich online.
Reverse Transcription Using ReadyScript cDNA Synthesis (RDRT)
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
Calcium Phosphate Transfection Kit Protocol
Calcium Phosphate Transfection Kit Protocol
Klenow Enzyme Protocol
Klenow Enzyme Protocol
Simplicon™ RNA Transfection and Electroporation Protocols
Simplicon™ RNA Transfection and Electroporation Protocols
Restriction Enzyme Cloning Manual Buffer Recipes
TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer
OverExpress™ Chemically Competent Cells
Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024
Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL
Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL
x-tracta Gel Extraction Tool
A quick, clean and easy to use alternative to using a scalpel or razor blade for the extraction of DNA and RNA bands from agarose gels following gel electrophoresis.
Blue-White Select™ Screening Reagent
Blue/white color selection is a routine technique employed by molecular biologists. This technique simplifies the differentiation between colonies/plaques that contain a cloning vector without an insert and those that contain a vector harboring an insert of interest.
Ligation of Insert to pGEX DNA
This page describes ligation of insert to pGEX DNA using GE Healthcare products.
Lipid Transfection Optimization Protocol
Lipid Transfection Optimization Protocol
X-tremeGENE™ 9 DNA Transfection Reagent Protocol
Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Selecting Correctly Expressing Recombinants
Blue White Screening; DNA Minipreps; Screening by Restriction Digestion; Screening by PCR; Confirm cut plasmid sizes by agarose gel electrophoresis; DNA Maxipreps; DNA Precipitation; RNase Treatment; Clean-up of DNA
OverExpress™ Electrocompetent Cells
OverExpress™ Electrocompetent Cells are provided in 25 μL aliquots, sufficient for one transformation reaction.
T4 DNA Ligase
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single-stranded nicks in duplex DNA...
DNA Ligation Protocol
The cloning process requires the ligation of linear DNA into a cloning vector. This ability to join fragments of DNA through recombinant technology is essential for many basic experiments in biotechnology.
Escort™ III Transfection Reagent Protocol
Escort™ III Transfection Reagent Protocol
QuickLink™ DNA Ligation Kit
T4 DNA ligase is used for the joining of DNA molecules with compatible cohesive (sticky) termini, joining of blunt ended double stranded DNA molecules
T4 DNA Ligase (Rapid) Protocol
T4 DNA Ligase for joining blunt ends of DNA and RNA.
Rapid DNA Ligation Kit Protocol
Rapid DNA Ligation Kit Protocol
Electroporation Protocol
This page describes electroporation using GE Healthcare products.
Alkaline Phosphatase (AP) Protocol
Alkaline Phosphatase (AP) Protocol
Alkaline Phosphatase Protocol
Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.