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Shengyuan Xiao et al.
Analytical biochemistry, 384(1), 20-26 (2008-10-11)
Methylamine and aminoacetone are endogenous aliphatic amines found in human blood and urine. They can be oxidized by semicarbazide-sensitive amine oxidase (SSAO), leading to the production of toxic aldehydes such as formaldehyde and methylglyoxal as well as hydrogen peroxide and
Ying Liu et al.
Journal of chromatographic science, 47(10), 925-930 (2009-11-26)
A sensitive and reliable method has been developed to determine synephrine in a famous purgative Chinese medicinal prescription, da-cheng-qi decoction. After purified by solid-phase extraction (SPE), the decoction was derivatized with 9-fluorenylmethyl chloroformate in borate buffer and analyzed by reversed-phase
J.V. Sancho et al.
Journal of Chromatography A, 678, 59-59 (1994)
K C Chan et al.
Journal of chromatography. A, 653(1), 93-97 (1993-10-29)
Laser-induced fluorescence (LIF) was applied to the detection of 9-fluorenylmethyl chloroformate (FMOC-Cl) derivatized amino acids separated by capillary electrophoresis. Fluorescence excitation was provided by a pulsed, KrF laser operating at 248 nm. A limit of detection of 5 x 10(-10)
Guoying Cao et al.
The Journal of antimicrobial chemotherapy, 62(5), 1009-1014 (2008-09-04)
The purpose of this study was to develop a specific, sensitive, accurate and reproducible high-performance liquid chromatographic (HPLC) method to measure polymyxin B in human plasma. Derivatization of polymyxin B with fluorescent 9-fluorenylmethyl chloroformate (FMOC-Cl) was performed in the same
K Puzio et al.
Journal of chromatography. A, 1361, 1-8 (2014-08-26)
Three molecularly imprinted polymers (MIPs) have been synthesized in order to bind efficiently glyphosate (GLY) in natural waters (mineral and underground). Since the target analyte is polar and hydrophilic, electrostatic interactions and hydrogen bonds have been favored with two templates
Bart Hens et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 63, 233-242 (2014-07-30)
The purpose of this study was to explore the transfer of drug solutions from stomach to small intestine and its impact on intraluminal drug concentrations in humans. The collected intraluminal data were used as reference to evaluate simulations of gastrointestinal
Jiawen Yu et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 25(7), 855-859 (2009-07-18)
A simple and sensitive reversed-phase liquid chromatographic method, based on the precolumn derivatization with 9-fluorenylmethyl chloroformate, was developed for the determination of myriocin. The derivatization reaction was performed in organic solvents of pyridine and tetrahydrofuran at 40 degrees C. Several
Mei-Fen Xie et al.
Journal of separation science, 34(3), 241-246 (2011-01-27)
In this study, we investigated a simple, sensitive and reliable liquid chromatography-fluorescence detection method for the determination of memantine hydrochloride in rat plasma which was based on derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl). For the first time, FMOC-Cl was introduced into
Yang Li et al.
Macromolecular bioscience, 15(1), 52-62 (2014-12-06)
To address the downside of conventional photo-patterning which can alter the chemical composition of protein scaffolds, we developed a non-covalent photo-patterning strategy for gelatin (denatured collagen) hydrogels that utilizes UV activated triple helical hybridization of caged collagen mimetic peptide (caged
Kun Qian et al.
Analytica chimica acta, 635(2), 222-226 (2009-02-17)
A pre-column derivatization high-performance liquid chromatographic method for glyphosate analysis has been developed. Derivatization of glyphosate was performed with 4-chloro-3,5-dinitrobenzotrifluoride (CNBF). In pH 9.5 H(3)BO(3)-Na(2)B(4)O(7) media, the reaction of glyphosate with CNBF completed at 60 degrees C for 30min. The
Y Zhang et al.
IET nanobiotechnology, 6(2), 76-80 (2012-05-09)
With the wide application of nanomaterials, the quantification of functional groups on nanomaterial surface becomes more and more necessary. A heterogeneous 9-fluorenylmethoxy carbonyl chloride (Fmoc-Cl) fluorescent method using an aqueous solution was established to determinate amino groups on nanomaterial surface.
H Brückner et al.
Amino acids, 42(2-3), 877-885 (2011-07-30)
The reagent 9-fluorenylmethoxycarbonyl chloride (Fmoc-Cl) was used for the pre-column derivatization of the biogenic amines (BAs) cadaverine (Cad), histamine (Him), octopamine (Ocp), phenylethylamine (Pea), putrescine (Put), spermidine (Spd), spermine (Spm), tyramine (Tym) and the internal standard 1,6-diaminohexane (Dhx). The resulting
En-Ping Lin et al.
Talanta, 114, 297-303 (2013-08-21)
This study proposes a sensitive method for the simultaneous separation and concentration of 9 pairs of amino acid enantiomers by combining poly(ethylene oxide) (PEO)-based stacking, β-cyclodextrin (β-CD)-mediated micellar electrokinetic chromatography (MEKC), and 9-fluoroenylmethyl chloroformate (FMOC) derivatization. The 9 pairs of
Yiqiang Chen et al.
Journal of separation science, 32(21), 3620-3626 (2009-10-21)
A sensitive and robust HPLC method was developed for the determination of kanamycin (Kan) in swine tissues. Kan was extracted from tissue with 10% TCA (w/v) solution and cleaned up with MCX SPE column. The purified extract was derivatized with
J. Kirschbaum et al.
Journal of Chromatography A, 661, 193-193 (1994)
G Csikó et al.
Journal of veterinary pharmacology and therapeutics, 37(4), 406-412 (2014-03-19)
Butyrate, a commonly applied feed additive in poultry nutrition, can modify the expression of certain genes, including those encoding cytochrome P450 (CYP) enzymes. In comparative in vitro and in vivo experiments, the effect of butyrate on hepatic CYP genes was
Xuezheng Song et al.
Analytical biochemistry, 395(2), 151-160 (2009-08-25)
Glycan microarray technology has become a successful tool for studying protein-carbohydrate interactions, but a limitation has been the laborious synthesis of glycan structures by enzymatic and chemical methods. Here we describe a new method to generate quantifiable glycan libraries from
Carlo Siciliano et al.
The Journal of organic chemistry, 77(23), 10575-10582 (2012-11-14)
The study describes a new "one-pot" route to the synthesis of N-9-fluorenylmethyloxycarbonyl (Fmoc) α-amino diazoketones. The procedure was tested on a series of commercially available free or side-chain protected α-amino acids employed as precursors. The conversion into the title compounds
Sourav Majumdar et al.
Biochimie, 103, 50-60 (2014-04-17)
In this study, biochemical and pharmacological characterization of Brevithrombolase, a fibrinolytic serine protease purified from Brevibacillus brevis strain FF02B has been reported. An assessment of its thrombolytic potency has also been made. The molecular mass of this monomeric protease was
A Korös et al.
Journal of chromatography. A, 1203(2), 146-152 (2008-08-05)
A high-performance liquid chromatographic method (HPLC) with combined diode array and fluorescence detection of amino acids and amines in various cheese samples is described. The proposal is based on acidic deproteinization, derivatization and gradient optimization studies, resulting in the identification
Chengke Li et al.
Methods in molecular biology (Clifton, N.J.), 970, 307-317 (2013-01-04)
Macrocyclic antibiotics, especially vancomycin, represent a class of versatile chiral selectors for enantioseparations by capillary electrophoresis (CE). In this chapter, we describe the protocol for performing CE enantioseparations with vancomycin as the chiral selector. Dynamic coating of the capillary with
S. Einarsson et al.
Analytical Chemistry, 58, 1638-1638 (1986)
Angela Patti et al.
Chirality, 20(2), 63-68 (2007-11-15)
The HPLC enantioseparation of the last generation antidepressive drug milnacipran (+/-)-1 was investigated on different cellulose-based chiral stationary phases (CSPs). On carbamate-type columns, Chiralcel OD and OD-H (+/-)-1 could be separated with alpha value about 1.20 but the resolution was
Bahareh Mohammadi et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 928, 32-36 (2013-04-20)
Topiramate is an antiepileptic agent, which is structurally different from the other anticonvulsants. The drug has no UV-Vis absorption or emits fluorescence. Thus for its analysis using high performance liquid chromatography (HPLC) with conventional UV or fluorescence detectors, the drug
Päivi Uutela et al.
Analytica chimica acta, 633(2), 223-231 (2009-01-27)
The efficiencies of three derivatisation reagents that react with either the amine (9-fluorenylmethyl chloroformate (FMOC)) or the carboxylic acid group (butanol) of amino acid or with both types of functional groups (propyl chloroformate) were compared in the analysis of amino
Miyako Nakano et al.
Glycobiology, 19(2), 135-143 (2008-10-29)
It is well known that most protein therapeutics such as monoclonal antibody pharmaceuticals and other biopharmaceuticals including cancer biomarkers are glycoproteins, and thus the development of high-throughput and sensitive analytical methods for glycans is essential in terms of their determination
Yang Chen et al.
Analytical and bioanalytical chemistry, 399(7), 2503-2509 (2011-01-19)
Functionalization of the surfaces of silica particles is often the first step in their various applications. An improved heterogeneous Fmoc-Cl fluorescent assay using an aqueous solution was developed to detect the number of amino groups on solid-phase supports. The fluorescent
Sary El Daker et al.
PloS one, 10(4), e0123772-e0123772 (2015-04-17)
Tuberculosis (TB) is still the principal cause of death caused by a single infectious agent, and the balance between the bacillus and host defense mechanisms reflects the different manifestations of the pathology. The aim of this work was to study
Qiong-Hui Zou et al.
Journal of separation science, 30(16), 2647-2655 (2007-09-18)
A novel approach for simultaneous determination of 12 sulphonamides (sulphadiazine, sulphamethazine, sulphathiazole, sulphadimethoxine, sulphamerazine, sulphapyridine, sulphamethoxazole, suphamethizole, sulphaquinoxaline, sulphameter, sulphamonomethoxine, and sulphachloropyridazine) in animal tissues (swine muscle and liver, chicken muscle, beef muscle) by HPLC with UV detection has been
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