Custom & Predesigned DNA Oligos and qPCR Probes
Oligonucleotides form the foundation of modern life science research, from basic genomic studies to disease diagnosis and therapeutics. In addition to their use as a standard or high-throughput primers in PCR, oligo nucleotides are used as probes, in microarray, in binding, in Immunostimulatory assays, in Sanger Sequencing & NGS (non-adapters), antisense analyses, and X-ray crystallographic structure determination.
As a supplier to the global life science industry of custom and predesigned DNA oligo products with more than 35 years of experience, we possess cutting-edge technology, competitive prices, and continuous investment in worldwide operations. We excel in difficult constructs with guaranteed quality and performance because of our comprehensive understanding of bioinformatics, various synthesis chemistries, and proprietary in-house engineered platforms. We perform regular quality checks using MALDI-TOF and Electrospray Ionization (ESI) Mass Spec and OD by UV spectroscopy and maintain a robust quality management system (QMS).
Product | Product Description | |
DNA Oligos in Tube | Non-modified and Modified oligos for applications including PCR, sequencing and cloning | Order |
DNA Oligos in Plates | Supplied in 96 or 384-well plates including many formatting options | Order |
Long Oligos | DNA oligo lengths from 121 to 180 bases for gene construction, microarray development and CRISPR gene editing | Order |
Easy™ Oligos | Normalized to 100 µM at a fixed price | Order |
Pure & Simple Primers | Cartridge purified primers at a fixed price | Order |
WellRED Oligos | For use with Beckman Coulter’s CEQ/GeXP Genetic Analysis Systems | Order |
iScale Oligos™ (mg & g quantity) | Larger quantities for in vivo, high throughput and commercial projects | Order |
Next-Gen Sequencing Oligos | Custom universal and index (barcode) adapter oligos for major sequencing platforms | Order |
Commercial Oligos | Custom DNA Primers, qPCR Probes, and Next-Gen Sequencing Oligos for Life Science Research Tools, Molecular Diagnostics (ISO 13485 manufacturing available), and Laboratory Developed Tests | Request Quote |
KiCqStart® Primers | Predesigned primer pairs for quantifying gene expression by SYBR® Green I RT-qPCR | Order |
KiCqStart® Primers Gene Arrays | Predesigned primer pairs organized into the groups of genes that comprise common gene families | Order |
KiCqStart® Probe Assays | Predesigned probe assays for quantifying gene expression by RT-qPCR | Order |
Dual-Labeled Probes | The most common probe type, linear hydrolysis probes are used in the 5’ nuclease assay | Order |
Molecular Beacons | Structured hybridization probes that are not degraded during the amplification reaction | Order |
LightCycler® Probes | Dual-hybridization probes designed for use in Roche™ LightCycler instruments | Order |
Scorpions® Uni-Probes | Combination one-part structured probe - primers that internally hybridize to the target sequence | Order |
Specifications for Custom & Predesigned DNA Oligos and qPCR Probes
Specifications | |
---|---|
Scale | 0.025, 0.05, 0.2, 1.0, 10 and 15 μmole |
Purification | Desalt, Cartridge, HPLC, PAGE |
Length | 10–120 bases, long oligo synthesis up to 180 bases |
Backbone | Phosphodiester / Phosphorothioate |
Common Modifications | Amine, Thiol, Phosphorylation, PEG Spacers, 2’-OMe-RNA, Biotin, etc. |
Fluorescent Dyes | 6-FAM™, TAMRA™, JOE™, TxRd (Sulforhodamine 101-X), WellRED (D2, D3 & D4), HEX™, TET™, Cyanine dyes |
Non-fluorescent Dyes | BHQ1™, BHQ2™, Dabcyl |
Quality Control | Every oligo verified using mass spectrometry, OD by UV spectroscopy |
Documentation | Certificate of Analysis including yield, Tm (melting temperature), MW, and μg/OD |
Format | Dry or In Solution (normalized) |
Packaging | 2-50 mL tubes, microtiter plates |
Oligos in Plates for High-Throughput (HTP) Applications
Whether your application requires a few or more than 100,000 oligos, we can provide the format to best suit your application, automation, and LIMS system. Our oligo experts can recommend the right plate for your system.
We specialize in liquid-handling systems to normalize oligonucleotide concentrations within a narrow range. Our investment in automation enables quick delivery of customer-specified products, no matter how complex. Shipping validation studies are conducted on all plate and seal combinations.
iScale Oligos™ DNA
iScale Oligos are perfect for in vivo, high-throughput, and diagnostic projects for which milligram and gram quantities of Custom DNA Oligos are required. These DNA oligos produced in State-of-the-art laboratories have high purity and precision, backed by a robust Quality Management System (QMS) and full documentation.
Custom Next-Generation Sequencing Oligos (NGSO)
Custom universal and index adapter sequences are required for major sequencing platforms. Our custom Next-Generation Sequencing Oligos (NGSO) are manufactured under rigorous conditions to ensure suitable purity and low cross-contamination to meet the research, commercial, and molecular diagnostic needs.
Predesigned Primers & qPCR Probes
KiCqStart® Primers, Gene Arrays, and Probe Assays
We offer a family of predesigned primers and probe assays for gene expression analysis. KiCqStart® Primers are predesigned SYBR® Green I Primers for two-step and one-step RT-qPCR. To comply with MIQE, they have been developed using sophisticated bioinformatics tools and validated in silico to avoid off-target amplification. Available for a variety of species and gene families, custom PCR primers, primer pairs, and assays can be easily searched and ordered online.
Real-time qPCR Probes
We offer a large probe chemistry selection for your RTqPCR needs. Our comprehensive portfolio of probes includes Dual-Labeled Probes, Molecular Beacons, LightCycler® Probes, Scorpions® Probes, and Locked Nucleic Acids® (LNA®)-containing Probes. Our probes are provided in a format that simplifies your experimental planning.
Real-time PCR Assay Design
OligoArchitect™ Primer and Probe Design Services
We are pleased to offer OligoArchitect™ services for all your primer and probe design requirements. OligoArchitect™ includes both our complimentary online design tool and our unique consultative service.
Explore our Custom Oligos & qPCR Probes Online Tools.
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