ZRB2168

Anti-SMCR8 Antibody, clone 1B3 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Guanine nucleotide exchange protein SMCR8
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1B3, recombinant monoclonal
• description: 1B3 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 105.02 kDa; observed mol wt ~35 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): ELISA: suitable; affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_658988
• UniProt accession no.: Q8TEV9
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... SMCR8(140775)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1B3 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects SMCR8. It targets an epitope within 15 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal half of human Guanine nucleotide exchange protein SMCR8.

Application

Quality Control Testing

Evaluated by Western Blotting with His-ABP-tagged recombinant fragment of Human SMCR8.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected His-ABP-tagged recombinant fragment of Human SMCR8.

Tested Applications

Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected His-ABP-tagged recombinant fragment of Human SMCR8.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SMCR8 in Caco-2 cells.

Affinity Binding Assay: A representative lot of this antibody bound SMCR8 peptide with a KD of 2.0 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Guanine nucleotide exchange protein SMCR8 (UniProt: Q8TEV9; also known as Smith-Magenis syndrome chromosomal region candidate gene 8 protein) is encoded by the SMCR8 gene (Gene ID: 140775) in human. Long isoform of human C9orf72 protein is known to form a complex with SMCR8 and WDR41 (WD Repeat domain 41) proteins. This complex displays guanine nucleotide exchange factor (GEF) activity and regulates autophagy. The N-terminal sequence (aa 1-349) of SMCR8 is required for its association with C9orf72.  In the complex, C9orf72 and SMCR8 are suggested to constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound Rab8A and Rab39B into their active GTP-bound form, thereby promoting autophagosome maturation. The C9orf72-SMCR8 complex is also reported to act as a negative regulator of autophagy initiation by interacting with the ULK1/ATG1 kinase complex and inhibiting its protein kinase activity. In addition to its activity in the cytoplasm within the C9orf72-SMCR8 complex, SMCR8 is also shown to localize in the nucleus, where it associates with chromatin and negatively regulates expression of suppresses ULK1 and WIPI2 genes. It is also reported to act as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Goodier, JL., et al. (2020). Acta Neuropathol. Commun. 8; Article 110; Jung, J., et al. (2017). Elife. 6; e23063; Sellier, C., et al. (2016). EMBO J. 35(12); 1276–1297; Sullivan, PM., et al. (2016). Acta Neuropathol. Commun. 4(1); Article 51).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable