ZRB1488

Anti-Cyclin E Antibody, clone 1C7 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Anti-Histamine Receptor 4 Antibody, clone 1E18 ZooMAb^® Rabbit Monoclonal, G1/S-specific cyclin-E1
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1C7, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 47.08 kDa; observed mol wt ~47 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal
• Protein ID accession no.: NP_001229
• UniProt accession no.: P24864
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... CCNE1(898)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1C7 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Cyclin E. It targets an epitope within 21 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal region of human Cyclin E.

Application

Quality Control Testing

Evaluated by Western Blotting in K562 cell nuclear extract.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Cyclin E in K562 cells nuclear extract.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Cyclin E in Human placenta tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cyclin E in K562 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Cyclin E (UniProt: P24864; also known as G1/S-specific cyclin-E1) is encoded by the CCNE1 (also known as CCNE) gene (Gene ID: 898) in human. Cyclin E is a nuclear protein that is essential for the control of the cell cycle at the G1/S transition. Its high expression is observed in testis and placenta and lower expression is observed in bronchial epithelial cells. The major role of cyclin E is to promote the assembly of the prereplication complex, through the loading of Cdc6 to the origins of replication and recruitment of the minichromosome maintenance protein complex (Mcm2-7), during the transition from quiescent G0 to G1 phases of the cell cycle and subsequently to trigger replication origin to allow cells to enter S phase. Cyclin E interacts with CDK2 to form a serine/threonine kinase holoenzyme complex where the cyclin subunit imparts substrate specificity to the complex. It serves a regulatory function and its binding to CDK2 activates the enzyme that can then phosphorylate severa

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable