ZRB1018

Anti-LEAP2 Antibody, clone 1D17 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Liver-expressed antimicrobial peptide 2
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1D17, recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 8.81 kDa; observed mol wt ~10 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): rat, mouse
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Internal
• Protein ID accession no.: NP_443203
• UniProt accession no.: Q969E1
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... LEAP2(116842)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D17 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects LEAP2. It targets an epitope within 14 amino acids from the internal region.

Immunogen

KLH-conjugated linear peptide corresponding to 14 amino acids from the internal region of human LEAP2.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant Human LEAP2.

Western Blotting Analysis (WB): 1:1,000 dilution of this antibody detected recombinant Human LEAP2.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LEAP2 in human skeletal muscle.

Affinity Binding Assay: A representative lot of this antibody bound LEAP2 peptide with a KD of 7.0 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected LEAP2 in human skeletal muscle tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Liver-expressed antimicrobial peptide 2 (UniProt: Q969E1; also known as LEAP-2) is encoded by the LEAP2 gene (Gene ID: 116842) in human. Cationic antimicrobial peptides are widely distributed in nature and have a key role in innate immunity and host defense and induce the direct destruction of microorganisms. LEAP2 is a 40-residue cationic antimicrobial peptide that is highly conserved in mammals. LEAP-2 is mainly expressed in the liver but is also produced in smaller quantities in other tissues. It is synthesized with a signal peptide (aa 1-22) and a propeptide (aa 23-37) that are subsequently cleaved off to generate the active mature peptide (aa 38-77). Its primary structure and disulfide bond pattern does not exhibit any similarity to any known peptide. It has a compact central core with disorder at the N- and C-termini. The core comprises a β-hairpin and a 3(10)- helix that are braced by disulfide bonds between Cys17-28 and Cys23-33 (in mature peptide) and is further stabilized by a network of hydrogen bonds. Its expression appears to be well regulated at the transcriptional level. LEAP2 gene consists of three exons and two introns. LEAP2 exhibits dose-dependent antimicrobial activity with an IC50 of about 5 μM against Saccharomyces cerevisiae and Bacillus subtilis. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Henriques, ST., et al. (2010). Chembiochem. 11(15); 148-157; Krause, A., et al. (2003). Protein Sci. 12(1); 143-152).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable