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Safety Information

SRP5086

Sigma-Aldrich

SLK, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

KIAA0204, LOSK, MGC133067, STK2, bA16H23.1, se20-9

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO® Kinase

Assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

3.9-5.3 nmol/min·mg

mol wt

~180 kDa

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... SLK(9748)

General description

SLK or LOSK is a Ste20-like protein kinase that associates with microtubule and centrosome. Inhibition of SLK activity by dominant-negative mutant or RNAi leads to unfocused microtubule arrangement indicating that SLK is needed for microtubule organization and for the proper localization of Golgi complex. Expression of v-Src can down-regulate SLK activity through the involvement of CK2 which can directly phosphorylate and inhibit SLK activity whereas inhibition of CK2 in v-Src-transformed cells results in normal SLK activity. CK2 and SLK can be co-localized in fibroblasts spreading on fibronectin-coated substrates.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Cartagena Act

Cartagena Act Listed

JAN Code

SRP5086-10UG:


Certificates of Analysis (COA)

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LOSK (SLK) protein kinase activity is necessary for microtubule organization in the interphase cell centrosome.
A V Burakov et al.
Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections, 403, 317-319 (2005-12-20)
Ziad Chaar et al.
The Journal of biological chemistry, 281(38), 28193-28199 (2006-07-14)
We have previously shown that the Ste20-like kinase SLK is a microtubule-associated protein inducing actin stress fiber disassembly. Here, we show that v-Src expression can down-regulate SLK activity. This down-regulation is independent of focal adhesion kinase but requires v-Src kinase

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