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SAB4200107

Sigma-Aldrich

Anti-CHD4 antibody, Mouse monoclonal

clone 3F2/4, purified from hybridoma cell culture

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

3F2/4, monoclonal

form

buffered aqueous solution

mol wt

antigen ~250 kDa

species reactivity

human, mouse

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): suitable
western blot: 0.5-1.0 μg/mL using HeLa cell extracts

isotype

IgG2a

UniProt accession no.

Q14839

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CHD4(1108)

General description

Monoclonal Anti-CHD4 (mouse IgG2a isotype) is derived from the hybridoma 3F2/4 produced by the fusion of mouse myeloma cells and splenocytes from CD-1 mice immunized with a synthetic peptide derived from human chromodomain helicase DNA binding protein 4 (CHD4) conjugated to keyhole limpet hemocyanine (KLH). CHD4 is one of the subunits of Mi-2, an ATP-dependent helicase. It belongs to the sucrose non-fermentable (SNF2)/Rad54 helicase family of proteins. The CHD4 gene is mapped to human chromosome 12p13.31 and is highly conserved among animals and plants.

Immunogen

synthetic peptide derived from human CHD4, conjugated to KLH

Application

Monoclonal Anti-CHD4 antibody produced in mouse has been used in:
  • immunoblotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunoprecipitation

Biochem/physiol Actions

Chromodomain helicase DNA binding protein 4 (CHD4) interacts with metastasis-associated protein (MTA1/2), histone deacetylase 1 (HDAC1) and RbAp46 in the nucleosome remodelling and deacetylation (NuRD) complex. Interestingly, antibodies against CHD4 have been found to be involved in the onset of dermatomyositis (DM), an autoimmune disease which is a form of idiopathic inflammatory myopathy (IIM). Variation in the gene due to mutation is observed in endometrial cancer. CHD4 regulates homologous recombination and thus maintains genome stability.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SAB4200107-200UL:
SAB4200107-BULK:
SAB4200107-25UL:
SAB4200107-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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UV radiation regulates Mi-2 through protein translation and stability
Burd CJ
Test, 283(50), 34976-34982 (2008)
The human Mi-2/NuRD complex and gene regulation
Denslow SA and Wade PA
Oncogene, 26(37), 5433-5433 (2007)
Detection of recurrent cytogenetic aberrations in multiple myeloma: a comparison between MLPA and iFISH
Zang M, et al.
Testing, 6(33), 34276-34276 (2015)
CHD4 mutations promote endometrial cancer stemness by activating TGF-beta signaling
Li Y, et al.
American Journal of Cancer Research, 8(5), 903-903 (2018)
Jinhua Quan et al.
PloS one, 9(9), e108066-e108066 (2014-09-24)
Loss of the chromatin remodeling ATPase CHD5 has been linked to the progression of neuroblastoma tumors, yet the underlying mechanisms behind the tumor suppressor role of CHD5 are unknown. In this study, we purified the human CHD5 complex and found

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