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Sucrose Phosphorylase

recombinant, expressed in E. coli, lyophilized powder, ≥45 units/mg solid

Sucrose:orthophosphate α-D-glucosytransferase
CAS Number:
Enzyme Commission number:
MDL number:

Quality Level


expressed in E. coli


lyophilized powder

specific activity

≥45 units/mg solid

mol wt

56 kDa by SDS-PAGE

shipped in

wet ice

storage temp.


General description

Sucrose Phosphorylase belongs to glycoside hydrolase, GH13 family. It comprises of four domains with the glucose anomeric carbon-binding site and a glucoside-binding site. The active site residues include Asp192 and Glu232. It is majorly produced by bifidobacteria and lactic acid bacteria. The cross-linked sucrose phosphorylase aggregates is thermostable and could be exploited for industrial catalysis of glycosylation.


Sucrose Phosphorylase has been used in sucrose determination in wheat plant and in sucrose hydrogen production.
Sucrose phosphorylase has been used in a study to assess the enzymatic synthesis of stable, odorless, and powdered furanone glucosides. Sucrose phosphorylase has also been used in a study to investigate the novel transglucosylating reaction with carboxylic compounds.


250, 1000 units in glass bottle

Biochem/physiol Actions

Sucrose phosphorylase catalyzes the reversible conversion of sucrose (α-D-glucopyranosyl-1,2-β-D-fructofuranoside) and phosphate into D-fructose and α-D-glucose 1-phosphate.
Enzymatically converts sucrose to D-fructose and D-glucose 1-phosphate.

Unit Definition

One unit will produce 1.0 μmole of D-fructose from sucrose per min with the corresponding reduction of NADP to NADPH at pH 7.6, at 25 °C.

Physical form

Contains sucrose as stabilizer.


Health hazard

Signal Word


Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids



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