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Safety Information

R306-05N

Sigma-Aldrich

Rat Cardiac Fibroblasts: RCF, neonatal

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

rat heart (Sprague Dawley, neonatal)

Quality Level

100

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 42

morphology

fibroblast

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cardiovascular diseases

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.


Cardiac fibroblasts are the most prevalent cell type in the heart, making up 60-70 % of all cells. They are known to play key roles in the development of cardiac disease and hypertrophy. Rat Cardiac Fibroblasts from Cell Applications, Inc. provide an excellent model system to study many aspects of heart function and pathophysiology, including the regulation of cell growth, survival, inflammation, hormone secretion and metabolism.

Cell Line Origin

Heart

Application

human heart function, neointimal formation, vascular remodeling, myocardial repair, extracellular maintenance, signaling, toxicology, gene expression, structural support for cardiomycytes, synthesis of extracellular matrix, growth factors and cytokines

Components

Fibroblast Basal Medium that contains 10% FBS and 10% DMSO

Preparation Note

  • 2nd passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO
  • Can be cultured at least 8 population doublings

Subculture Routine

Please refer to the RCF Culture Protocol.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

R306-05N:

Certificates of Analysis (COA)

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Protocols

Rat Cardiac Fibroblasts (RCF) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

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