Skip to Content
Merck
All Photos(1)

Documents

Safety Information

N0513

Sigma-Aldrich

Nerve Growth Factor-7S from murine submaxillary gland

NGF-7S, lyophilized powder, suitable for cell culture

Synonym(s):

NGF-7S

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
93928-24-6
EC Number:
210-123-3
MDL number:
MFCD00147703
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

mouse

form

lyophilized powder

potency

2-250 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

protein 130 kDa

packaging

pkg of 0.1 mg

technique(s)

cell culture | mammalian: suitable

impurities

≤10

color

white

UniProt accession no.

P01139

storage temp.

−20°C

Gene Information

mouse ... Ngf(18049)

Related Categories

General description

Nerve growth factor (NGF) is a dimer of 118-residue polypeptide chains linked non-covalently. Both these polypeptides contain three intramolecular disulphide bridges. It forms the prototype of the neurotrophin growth factor family. In male mouse submandibular salivary gland, NGF is present in high amounts, and is present as a high molecular weight complex called nerve growth factor-7S (NGF-7S). NGF-7S is an α2β2γ2 complex, where β2-NGF is the active neurotrophin, and is linked to the dimers of the glandular kallikrein serine proteinases members, α-NGF and γ-NGF.

Application

Nerve Growth Factor-7S (NGF-7S) from murine submaxillary gland has been used for the treatment of following cells to induce neuronal differentiation-
  • PC12
  • Dorsal root ganglia (DRG) obtained from chick embryos of either sex (Gallus gallus)

Biochem/physiol Actions

Nerve growth factor (NGF) is a neurotrophin, which facilitates the differentiation and survival of specific subtypes of neurons of central nervous system and peripheral nervous system. However, nerve growth factor-7S (NGF-7S) lacks any neurotrophic activity as most part of β-NGF subunit is buried within the NGF-7S complex.

Physical form

Lyophilized from 0.2 μm filtered solution in 25 mM sodium phosphate, pH 7.0.

Preparation Note

Nerve growth factor-7S (NGF-7S) is isolated from male mouse submaxillary glands using a modification of the method of Varon, et al.

Analysis Note

The biological activity is measured using a 3-day MTT assay.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

N0411-1VL-PW:
N0513-BULK-N:
N0513-.1MG:
N0513-PH:
N0513-VAR:
N0513PROC:
N0513-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Kun-Shan Huang et al.
Evidence-based complementary and alternative medicine : eCAM, 2011, 109809-109809 (2009-08-19)
The present study provides in vitro and in vivo evaluation of Paeoniae alba Radix (PR) on peripheral nerve regeneration. In the in vitro study, we found the PR caused a marked enhancement of the nerve growth factor-mediated neurite outgrowth from
Hsien-Tung Chen et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 84(1), 256-262 (2007-05-22)
This study provides in vitro and in vivo evaluation of rat serum metabolites of the Pueraria lobata (SMP) on peripheral nerve regeneration. In the in vitro study, we found that the SMP caused a marked enhancement of the nerve growth
B Bax et al.
Structure (London, England : 1993), 5(10), 1275-1285 (1997-11-14)
Nerve growth factor (NGF) is a neurotrophic factor that promotes the differentiation and survival of certain populations of neurons in the central and peripheral nervous systems. 7S NGF is an alpha 2 beta 2 gamma 2 complex in which the
The isolation of the mouse nerve growth factor protein in a high molecular weight form.
S Varon et al.
Biochemistry, 6(7), 2202-2209 (1967-07-01)
Claudia Guldimann et al.
International journal of experimental pathology, 93(4), 259-268 (2012-07-19)
Central nervous system (CNS) infections in ruminant livestock, such as listeriosis, are of major concern for veterinary and public health. To date, no host-specific in vitro models for ruminant CNS infections are available. Here, we established and evaluated the suitability
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service