MAK034

Coenzyme A (CoA) Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 colorimetric or fluorometric tests
• detection method: colorimetric; fluorometric
• functional group: phospholipid
• relevant disease(s): endocrinological disorders, diabetes; cancer
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Coenzyme A (CoA) is an essential metabolic cofactor synthesized from cysteine, pantothenate, and ATP (adenosine triphosphate). CoA plays important roles in many metabolic pathways, including the Tricarboxylic Acid cycle, and the synthesis and oxidation of fatty acids. One of the main functions of CoA is the carrying and transfer of acyl groups. Acylated deriviates, for example Acetyl-CoA, are critical intermediates in many metabolic reactions, particularly fatty acid and carbohydrate metabolism. CoA levels can be altered during starvation, and in conditions such as cancer, diabetes, and alcoholism.

Application

Coenzyme A (CoA) Assay Kit has been used to measure CoA levels.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of Coenzyme A (CoA) in a variety of biological samples including cell and tissue lysates

Principle

The Coenzyme A Assay kit is an easy and convenient assay to measure the CoA level in a variety of biological samples. CoA concentration is determined by an enzymatic assay, which results in a colorimetric (570 nm)/fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the CoA present. Typical sensitivities of detection for this kit are 0.1-10 nmol of CoA.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H319,H334
• Precautionary Statements: P261 - P264 - P280 - P284 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup