These HiTrap columns are prepacked with MabSelect PrismA protein A chromatography resin. This affinity resin in the packed column has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein ligand, allowing efficient cleaning between monoclonal antibody purification runs. This allows future demands in monoclonal antibody purification to be met, including processing of many bispecific antibodies.
Enhanced dynamic binding capacity compared with other protein A resins.
Excellent alkaline stability enables efficient cleaning and sanitization using 0.5–1.0 M NaOH.
Convenient HiTrap format for easy connection to a syringe, peristaltic pump, or chromatography systems such as an ÄKTA system for convenient process optimization.
The enhanced alkaline stability of MabSelect PrismA also provides opportunities for more robust mAb purification using periodic counter-current chromatography. The possibility for cleaning using 1 M NaOH increases bioburden control for continuous processes, which can be exposed to days or weeks of feed harvest.