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F4143

Sigma-Aldrich

Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
MFCD00162628
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
indirect immunofluorescence: 1:128

storage temp.

−20°C

target post-translational modification

unmodified

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General description

IgG antibody is secreted by B cells and is found in blood and extracellular fluids and
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system.

Specificity

Binds mouse IgG; does not bind other mouse Igs.

Immunogen

purified mouse IgG, Fc fragment

Application

Anti-Mouse IgG (Fc specific)-FITC antibody has been used in
  • immunohistochemistry
  • immunofluorescence
  • flow cytometry
  • immunocytochemistry
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

IgG provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with bovine, horse or human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

F4143-VAR:
F4143-1ML:
F4143-2ML:
F4143-.5ML:
F4143-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jiří Zahradník et al.
ACS synthetic biology, 10(12), 3445-3460 (2021-11-24)
Here, we enhanced the popular yeast display method by multiple rounds of DNA and protein engineering. We introduced surface exposure-tailored reporters, eUnaG2 and DnbALFA, creating a new platform of C and N terminal fusion vectors. The optimization of eUnaG2 resulted
Qiong Wu et al.
Applied and environmental microbiology, 82(4), 1173-1182 (2015-12-15)
Escherichia coli is a major environmental pathogen causing bovine mastitis, which leads to mammary tissue damage and cell death. We explored the effects of the probiotic Lactobacillus rhamnosus GR-1 on ameliorating E. coli-induced inflammation and cell damage in primary bovine
P Xu et al.
Iranian journal of veterinary research, 22(1), 65-71 (2021-06-22)
Mammary epithelial cells (MECs) have been widely-used over the years as models to understand the physiological function of mammary disease. This study aimed to establish a culture system and elucidate the unique characteristics of bovine mammary epithelial cells (BMECs) from
Qiong Wu et al.
Frontiers in microbiology, 9, 1661-1661 (2018-08-09)
Escherichia coli is a common cause of mastitis in dairy cows. The adaptor protein apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) synergizes with caspase-1 to regulate inflammasome activation during pathogen infection. Here, the ASC gene was knocked out
Chiara Bernardini et al.
International journal of molecular sciences, 21(15) (2020-07-30)
Hydrogen sulfide (H2S) is now considered not only for its toxicity, but also as an endogenously produced gas transmitter with multiple physiological roles, also in maintaining and regulating stem cell physiology. In the present work, we evaluated the effect of
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