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EPI001

Sigma-Aldrich

Histone Acetyltransferase (HAT) Activity Assay Kit

100 assays in 96 well plates

Enzyme Commission number:
NACRES:
NA.41

Quality Level

usage

100 assays in 96 well plates

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

Histone acetyltransferases (HATs) have critical roles in cellular functions and are involved in the regulation of gene transcription, differentiation, and proliferation. The HAT Activity Colorimetric Assay Kit offers a convenient, non-radioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit includes an active Nuclear Extract (NE) to be used as a positive control, HAT cofactor, acetyl-CoA, and all downstream cofactors and substrates. Acetylation of a peptide substrate by active HAT releases the free form of CoA, which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous, and therefore, is suitable for kinetic studies. The kit provides all reagents and a simple, straightforward protocol for a complete assay.

Features and Benefits

  • Sensitive and reliable assay
  • Utilizes colorimetric methods
  • Sample type: cell and tissue lysates
  • Species reactivity: mammalian
  • Suitable for individual tests or high throughput assays and kinetic studies
  • Easy and simple procedure: combine nuclear extract or protein sample with reagents, incubate, and read absorbance
  • Convenient 96-well microplate format
  • Continuously measures HAT activity; suitable for kinetic studies
  • No HDAC interference; crude nuclear extract can be used directly

Storage Class Code

12 - Non Combustible Liquids

Certificate of Analysis

Certificate of Origin

Product Information Sheet

More documents

Quotes and Ordering

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Accumulating evidence indicates that epigenetic aberrations have a role in the pathogenesis of rheumatoid arthritis (RA). However, reports on histone modifications are as yet quite limited in RA. Interleukin (IL)-6 is an inflammatory cytokine which is known to be involved
Metformin inhibits androgen-induced IGF-IR up-regulation in prostate cancer cells by disrupting membrane-initiated androgen signaling.
Malaguarnera R, Sacco A, Morcavallo A, et al.
Endocrinology, 155(4), 1207-1221 (2014)
Qiao Zhang et al.
Science (New York, N.Y.), 343(6168), 298-301 (2014-01-18)
Ribosome biogenesis drives cell growth and proliferation, but mechanisms that modulate this process within specific lineages remain poorly understood. Here, we identify a Drosophila RNA polymerase I (Pol I) regulatory complex composed of Under-developed (Udd), TAF1B, and a TAF1C-like factor.
Si-Taek Oh et al.
FEBS letters, 588(5), 685-691 (2014-02-05)
We report that H3K9 HMTase G9a activates transcription of the cell cycle regulatory gene, p21, in p53-null H1299 cells. Positive regulation of p21 by G9a is independent of its HMTase activity. We demonstrate that G9a upregulates p21 via interaction with

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