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E5546

Sigma-Aldrich

Erythropoietin (EPO) human

recombinant, expressed in HEK 293 cells, suitable for cell culture

Synonym(s):

EPO, epoetin, hematopoietin, hemopoietin

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

recombinant

expressed in HEK 293 cells

Assay

≥98% (SDS-PAGE)

form

lyophilized powder

potency

≤2.5 ng/mL ED50 (measured in a cell proliferation assay using TF-1 human cells)

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

storage temp.

−20°C

General description

Recombinant human erythropoietin (EPO) is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of ~18 kDa. This protein is manufactured in human cells using an all-human production system, with full chemically defined ingredients and with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture. The protein is produced with no artificial tags.

Biochem/physiol Actions

Erythropoietin (EPO) is a glycoprotein hormone, known for its erythropoietic activity. It is used as treatment for anemia in humans with renal failure or cancer. EPO also reduces the amount of blood transfusions required on premature infants and surgeries.
EPO consists of 165 amino acids and has a high carbohydrate content, of both N-linked and O-linked types. The oligosaccharide chains are responsible for EPO production, secretion, longevity, and functioning. The biological activity of EPO depends upon two disulfide bonds, one formed between cysteine7 and cysteine160, and the other between cysteine29 and cysteine33. Thus, EPO proper folding, glycosylation and disulfide bonds formation are crucial for its function.
EPO was found to downregulate HIF-1α in the diabetic rat model. EPO helped maintaining the homeostasis of intracellular zinc in retinal cells, altered as a result of diabetes, by restoring Zinc transporter 8 (ZnT8) expression.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

E5546-BULK:
E5546-VAR:
E5546-50UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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