Skip to Content
Merck
All Photos(3)

Key Documents

View All Documentation

Safety Information

A7554

Sigma-Aldrich

Monoclonal Anti-Bovine IgG−Alkaline Phosphatase antibody produced in mouse

clone BG-18, purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):

Monoclonal Anti-Bovine IgG

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

BG-18, monoclonal

form

buffered aqueous glycerol solution

species reactivity

bovine (non-reactive to IgM)

should not react with

pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat

technique(s)

direct ELISA: 1:25,000
dot blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG
western blot (chemiluminescent): 1:100,000

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction.

Specificity

bovine IgG1 and IgG2

Immunogen

Purified bovine IgG

Application

Monoclonal Anti-Bovine IgG?Alkaline Phosphatase antibody produced in mouse has been used in:
  • western blotting
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • immunohistochemistry

Biochem/physiol Actions

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

IXO97634:
A7554-.5ML:
A7554-BULK:
A7554-.2ML:
A7554-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

E M Abdo et al.
Clinical and diagnostic laboratory immunology, 7(4), 588-595 (2000-07-07)
Lipoprotein LppQ, a predominant 48-kDa antigen, and its corresponding gene, lppQ, were characterized in Mycoplasma mycoides subsp. mycoides SC, the etiological agent of contagious bovine pleuropneumonia. The lppQ gene is specific to M. mycoides subsp. mycoides SC and was found
Genetic and immunologic analyses of PlpE, a lipoprotein important in complement-mediated killing of Pasteurella haemolytica serotype 1
Pandher K, et al.
Infection and Immunity, 66(12), 5613-5619 (1998)
Andreas Kurth et al.
American journal of veterinary research, 67(1), 114-119 (2006-01-24)
To examine sera obtained from dairy and beef cattle to detect antibodies against vesivirus and compare seroprevalence among cattle within the sample population. Cattle sera from 8 western states and Maryland submitted to the Washington Animal Disease Diagnostic Laboratory during
Haet Sal Jeon et al.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 27(3), 344-351 (2015-04-10)
Antibody responses are useful indicators of Mycobacterium bovis infection in cattle. Many studies have evaluated the ability of immunoglobulin G (IgG) to serodiagnose bovine tuberculosis (TB). In the current study, immunoglobulin A (IgA) and IgG responses against the MPB70 and
Pamela Nicholson et al.
Anaerobe, 56, 78-87 (2019-02-17)
Clostridium chauvoei is the etiologic agent of blackleg in cattle, inducing fever, severe myonecrosis, oedemic lesions and ultimately death of infected animals. The pathogen often results in such rapid death that antibiotic therapy is futile and thus vaccination is the
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service