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A5963

Sigma-Aldrich

Anti-c-Myc−Alkaline Phosphatase antibody, Mouse monoclonal

clone 9E10, purified from hybridoma cell culture

Synonym(s):

Anti-c-Myc, Monoclonal Anti-c-Myc−Alkaline Phosphatase antibody produced in mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9E10, monoclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: 1:100 using a myc-tagged fusion protein.

isotype

IgG1

UniProt accession no.

P01106

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... MYC(4609)

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General description

Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/c mouse. The c-myc gene encodes for a protein of 62kDa called p62c-myc is associated mainly with cell nuclei.

Immunogen

synthetic peptide of the human p62c-Myc protein.

Application

Anti-c-Myc-Alkaline Phosphatase antibody has been used in
  • enzyme linked immunosorbent assay (ELISA)
  • immunohistochemical labeling
  • immunostaining
  • western blotting

Monoclonal Anti-c-Myc-Alkaline Phosphatase antibody can be used for western blot assays at 1:100, using myc-tagged fusion proteins. The antibody can also be used for immunohistochemical applications.

Biochem/physiol Actions

The c-myc oncogene product(p62c-myc) exerts normal and oncogenic cellular functions. Elevated level of c-myc protein in malignant tissues when compared with normal tissue, but with the unexpected finding of a cytoplasmic accumulation of the protein in these tumors.

Physical form

Solution in 0.05 M Tris buffer, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A5963-.5ML:
A5963-VAR:
A5963-BULK:
A5963-.25ML:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Feng,L, et al.
Autophagy, 13(4), 686-702 (2017)
Novel ganglioside antigen identified by B cells in human medullary breast carcinomas: the proof of principle concerning the tumor-infiltrating B lymphocytes
Kotlan B, et al.
Journal of Immunology, 175(4), 2278-2285 (2005)
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
Evan G I, et al.
Molecular and Cellular Biology, 5(12), 3610-3616 (1985)
Beatrix Kotlan et al.
Journal of immunology (Baltimore, Md. : 1950), 175(4), 2278-2285 (2005-08-06)
The potential tumor-recognizing capacity of B cells infiltrating human breast carcinoma is an important aspect of breast cancer biology. As an experimental system, we used human medullary breast carcinoma because of its heavy B lymphocytic infiltration paralleled to a relatively
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