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D8537

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DPBS

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About This Item

MDL number:
MFCD00131855
UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.75

product name

Dulbecco′s Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Quality Level

300

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: no (Dextro)
sodium pyruvate: no

shipped in

ambient

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General description

Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS buffer is used to irrigate, wash, and dilute mammalian cells while maintaining osmotic balance. It provides water and inorganic ions essential for normal cell metabolism. DPBS with calcium and magnesium facilitates cell binding and clumping. Dulbecco′s PBS without these ions can be used to wash and rinse suspended cells. Phosphate buffering maintains the pH in the physiological range.

Application

Dulbecco′s Phosphate Buffered Saline has been used:

  • for the dilution of fresh red blood cells to study the trapping of red blood cell aggregate.
  • to wash the wells of a microtiter plate.
  • to wash the cells before resuspending them in lysis buffer for DNA isolation.
  • as a component of embryo holding medium for vitrification of embryos.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

D8537-VAR:
D8537-PROC:
D8537-10BB:
D8537-BULK:
D8537-24X500ML:
D8537PROC:
D8537-1L:
D8537-CH:
D8537-100ML:
D8537-1L-C:
D8537-500ML:
D8537-6X500ML:
D8537-500ML-C:
D8537-6X1L:
D8537-100ML-C:
D8537-PH:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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