Merck
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75977

Millipore

PALCAM Listeria Selective Agar

suitable for microbiology, NutriSelect® Plus

NACRES:
NA.85

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 13.0 g/L
ammonium ferric citrate, 0.5 g/L
esculin, 0.8 g/L
glucose, 0.5 g/L
lithium chloride, 15.0 g/L
D-mannitol, 10.0 g/L
peptone, 23.0 g/L
phenol red, 0.08 g/L
sodium chloride, 5.0 g/L
starch, 1.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

application(s)

agriculture
environmental
food and beverages
pathogen testing
veterinary

microbiology

suitability

selective and differential for Listeria spp.

General description

Polymyxin acriflavine lithium chloride ceftazidime aesculin mannitol (PALCAM) Listeria Selective Agar is a selective medium. Selectivity is attained mainly by the inhibitory components like lithium chloride, acriflavine, polymyxin and ceftazidime. Two indicator systems confer its diagnostic properties: aesculin and ferric iron and D-mannitol combined with phenol red. This aids in easy differentiation of Listeria spp. from enterococci and staphylococci.

Application

for differential diagnosis, for the isolation and detection of Listeria monocytogenes from fecal and biological material, food and heavily contaminated environmental specimens.

Preparation Note

Dissolve 68.8 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool down to 50°C and add the dissolved contents of 2 vials PALCAM Listeria Selective Supplement (Cat. No. 02336). The completed broth may appear hazy due to starch.

Other Notes

Detection of Listeria species in a range of meat products

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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