75977

PALCAM Listeria Selective Agar

suitable for microbiology, NutriSelect^® Plus

• NACRES: NA.85

Properties

• Quality Level: 200
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 13.0 g/L ; ammonium ferric citrate, 0.5 g/L ; esculin, 0.8 g/L ; glucose, 0.5 g/L ; lithium chloride, 15.0 g/L ; D-mannitol, 10.0 g/L ; peptone, 23.0 g/L ; phenol red, 0.08 g/L ; sodium chloride, 5.0 g/L ; starch, 1.0 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 7.0±0.2 (25 °C)
• application(s): agriculture; environmental; food and beverages; pathogen testing; veterinary; microbiology
• suitability: selective and differential for Listeria spp.

Description

General description

Polymyxin acriflavine lithium chloride ceftazidime aesculin mannitol (PALCAM) Listeria Selective Agar is a selective medium. Selectivity is attained mainly by the inhibitory components like lithium chloride, acriflavine, polymyxin and ceftazidime. Two indicator systems confer its diagnostic properties: aesculin and ferric iron and D-mannitol combined with phenol red. This aids in easy differentiation of Listeria spp. from enterococci and staphylococci.

Application

for differential diagnosis, for the isolation and detection of Listeria monocytogenes from fecal and biological material, food and heavily contaminated environmental specimens.

Preparation Note

Dissolve 68.8 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool down to 50°C and add the dissolved contents of 2 vials PALCAM Listeria Selective Supplement (Cat. No. 02336). The completed broth may appear hazy due to starch.

Other Notes

Detection of Listeria species in a range of meat products

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315 - H319
• Precautionary Statements: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves