67454

Bolton Broth, Base

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): Bolton Selective Enrichement Broth, Base
• NACRES: NA.85

Properties

• Quality Level: 200
• Agency: according to ISO 10272-1:2017
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: enzymatic digest of animal tissues, 10 g/L ; α-ketoglutaric acid, 1.0 g/L ; lactalbumin hydrolysates, 5.0 g/L ; sodium carbonate, 0.6 g/L ; sodium chloride, 5.0 g/L ; sodium metabisulfite, 0.5 g/L ; sodium pyruvate, 0.5 g/L ; yeast extract, 5.0 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 7.4±0.2 (25 °C)
• application(s): clinical testing; food and beverages; pathogen testing; sample preparation; microbiology
• suitability: selective for Campylobacter spp.

Description

General description

Bolton broth base is used for the selective enrichment of Campylobacter species from foods as recommended by ISO Committee under specifications ISO 10272-1:2006 (E).

Application

for the selective enrichment of Campylobacter species from foods

Preparation Note

Suspend 13.8 grams in 472.5 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50 °C and aseptically add rehydrated contents of 1vial of Bolton Selective Supplement (Cat. No. 40568) and 25 ml of sterile lysed defribinated horse blood. Mix well before dispensing into sterile tubes.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H317 - H318
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P305 + P351 + P338 - P333 + P313
• Hazard Classifications: Eye Dam. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves