Merck
All Photos(1)

67454

Millipore

Bolton Broth, Base

suitable for microbiology, NutriSelect® Plus

Synonym(s):
Bolton Selective Enrichement Broth, Base
NACRES:
NA.85

Quality Level

Agency

according to ISO 10272-1:2017

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

enzymatic digest of animal tissues, 10 g/L
α-ketoglutaric acid, 1.0 g/L
lactalbumin hydrolysates, 5.0 g/L
sodium carbonate, 0.6 g/L
sodium chloride, 5.0 g/L
sodium metabisulfite, 0.5 g/L
sodium pyruvate, 0.5 g/L
yeast extract, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.4±0.2 (25 °C)

application(s)

clinical testing
food and beverages
pathogen testing
sample preparation

microbiology

suitability

selective for Campylobacter spp.

General description

Bolton broth base is used for the selective enrichment of Campylobacter species from foods as recommended by ISO Committee under specifications ISO 10272-1:2006 (E).

Application

for the selective enrichment of Campylobacter species from foods

Preparation Note

Suspend 13.8 grams in 472.5 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50 °C and aseptically add rehydrated contents of 1vial of Bolton Selective Supplement (Cat. No. 40568) and 25 ml of sterile lysed defribinated horse blood. Mix well before dispensing into sterile tubes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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