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Key Documents

Safety Information

0065-FP

Sigma-Aldrich

3`-suLea-C3-PAA-fluo

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About This Item

UNSPSC Code:
12352201
NACRES:
NA.25

carrier

PAA carrier

label

FITC labeled

product line

FP-Series

Assay

95% (H-NMR)

form

lyophilized powder

mol wt

~20,000 Da

manufacturer/tradename

GlycoNZ

extent of labeling

1% Biotin per 20 mole % Poly[N-(2-hydroxyethyl)acrylamide-co-N-acrylamide] ligand density

technique(s)

flow cytometry: suitable

probe type

trisaccharide

probe presentation

polymeric

storage temp.

−20°C

General description

FP-Series: Fluorescein-Labeled Polymeric Probes
These glycoconjugate probes are designed to reveal lectins, including selectins, directly on cell membranes, organelles, and in cytoplasm. The low fluorescein content ensures that probe solubility in water is not affected, but still allows reliable probe detection. The affinity of the probes is 102 to 105 times higher than that of corresponding free sugars.

PAA is poly[N-(2-hydroxyethyl)acrylamide], a flexible polymer chain which serves as an additional spacer. Used as a molecular weight carrier, PAA demonstrates an absence of non-specific interaction with cells, and is stable to chemical and proteolytic action.

Application

Glycoconjugates are used to study the functions of carbohydrates, carbohydrate-binding proteins in biological processes, and on the discovery of new carbohydrate-mediated interactions.

Preparation Note

Purity: synthetic ω-aminoalkyl glycosides used for coupling with the polymer have a purity of >95% (HPLC and 1H-NMR)

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

GNZ-0065-FP:

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