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MABC006F

Sigma-Aldrich

Mouse IgG2b Negative Control, clone GC198, FITC conjugate

Mouse IgG2b Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.42
conjugate:
FITC conjugate
application:
FACS
IF
clone:
GC198, monoclonal
technique(s):
flow cytometry: suitable
immunofluorescence: suitable
citations:
9

biological source

mouse

Quality Level

100

conjugate

FITC conjugate

antibody form

purified immunoglobulin

clone

GC198, monoclonal

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunofluorescence: suitable

isotype

IgG2b

shipped in

wet ice

target post-translational modification

unmodified

Specificity

This antibody reacts with the outer membrane protein of some strains of Neisseria gonorrhoeae. No reaction with human cell surface or plasma components has been observed. However, some binding to Fc receptors may occur.

Immunogen

Protein I preparation from Neisseria gonorrhoeae.

Application

FITC conjugated mouse IgG2b is a monoclonal antibody intended for use as a negative control in flow cytometry. Its use enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.

SUGGESTED USAGE

Flow cytometry - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
Mouse IgG2b Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.
Research Category
Secondary & Control Antibodies

Epitope Tags & General Use
Research Sub Category
Isotype Control Antibodies

Physical form

Purified immunoglobulin. Fluorescein conjugated, ready to use. The antibody is supplied in 1.0ml phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.

Storage and Stability

Store at 2 to 8oC, for up to 6 months. DO NOT FREEZE. Protect from light.

WARNING

The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MABC006F:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Narges Labibzadeh et al.
Cell journal, 18(3), 302-309 (2016-09-08)
Nonunion is defined as a minimum of 9 months since injury without any visible progressive signs of healing for 3 months. Recent literature has shown that the application of mesenchymal stromal cells is safe, in vitro and in vivo, for
Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions.
Beers, J; Gulbranson, DR; George, N; Siniscalchi, LI; Jones, J; Thomson, JA; Chen, G
Nature Protocols null
Vivek Shukla et al.
Cancer research, 77(22), 6267-6281 (2017-09-25)
In this study, we generated induced pluripotent stem cells (iPSC) from normal human small airway epithelial cells (SAEC) to investigate epigenetic mechanisms of stemness and pluripotency in lung cancers. We documented key hallmarks of reprogramming in lung iPSCs (Lu-iPSC) that

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