AP500R

Goat Anti-Mouse IgM µ chain Antibody, Rhodamine conjugate, Species Adsorbed

1 mg/mL, Chemicon^®

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.46

Properties

• biological source: goat
• Quality Level: 100
• conjugate: TRITC conjugate; rhodamine conjugate
• antibody form: affinity purified immunoglobulin
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: mouse
• manufacturer/tradename: Chemicon^®
• concentration: 1 mg/mL
• technique(s): ELISA: suitable; immunofluorescence: suitable
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

IgM constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as ′natural′ blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences.

Specificity

Reacts with heavy chain of mouse IgM. Absorbed for mouse IgG1, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins. Reactivity with other species has not been determined.

The antibody reacts with the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Immunogen

Epitope: IgM µ chain

Prepared from purified mouse IgM µ chain. Pooled antisera from goats hyperimmunized with mouse IgM paraproteins.

Application

Detect Mouse IgM µ chain using this Goat anti-Mouse IgM µ chain Antibody, Rhodamine conjugate, Species Adsorbed validated for use in ELISA & IF.

Immunofluorescence: ≤1 μg/10⁶ cells.
ELISA

Optimal working dilutions must be determined by the end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Fragment Specific Secondary Antibodies

Physical form

Affinity Chromatography

Purified by affinity chromatography on mouse IgM covalently linked to agarose. Liquid in 1.0 mL PBS/NaN³.

Storage and Stability

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Do not freeze.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable