235423-M

Caspase-3 Inhibitor I, Cell-Permeable

The Caspase-3 Inhibitor I, Cell-Permeable controls the biological activity of Caspase-3. This small molecule/inhibitor is primarily used for Cancer applications.

• Synonym(s): Caspase-3 Inhibitor I, Cell-Permeable, CPP32/Apopain Inhibitor, Cell-permeable, Cell-permeable, DEVD-CHO
• Empirical Formula (Hill Notation): C₉₄H₁₅₈N₂₀O₂₇
• Molecular Weight: 2000.38
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥97% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: DMSO: 5 mg/mL
• storage temp.: −20°C

Description

General description

A cell-permeable inhibitor of caspase-3, as well as caspase-6, caspase-7, caspase-8, and caspase-10. The C-terminal DEVD-CHO sequence of this peptide is a highly specific, potent, and reversible inhibitor of caspase-3 (K_i<1 nM) that has also been shown to strongly inhibit PARP cleavage in cultured human osteosarcoma cell extracts (IC₅₀ = 200 pM). The N-terminal sequence (amino acid residues 1-16) corresponds to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF) and confers cell-permeability to the peptide. A 5 mM (1 mg/100 l) solution of Caspase-3 Inhibitor I, Cell-permeable in DMSO is also available.

A cell-permeable inhibitor of caspase-3. The C-terminal DEVD-CHO sequence of this peptide is a highly specific, potent and reversible inhibitor of caspase-3 (K_i<1 nM) that has also been shown to strongly inhibit PARP cleavage in cultured human osteosarcoma cell extracts (IC₅₀ = 0.2 nM). The N-terminal sequence (residues 1-16) corresponds to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF) and confers cell-permeability to the peptide.

Biochem/physiol Actions

Primary Target
caspase-3

Target IC₅₀: 200 pM for inhibiting PARP cleavage in cultured human osteosarcoma cell extracts

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Asp-Glu-Val-Asp-CHO

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Other Notes

Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Lin, Y.-Z., et al. 1996. J. Biol. Chem. 271, 5305.
Nicholson, D.W. 1996. Nature Biotechnol. 14, 297.
Schlegel, J., et al. 1996. J. Biol. Chem.271, 1841.
Lin, Y.-Z., et al. 1995. J. Biol. Chem.270, 14255.
Nicholson, D.W., et al. 1995. Nature 376, 37.
Tewari, M., et al. 1995. Cell 81, 801.
Lazebnik, Y.A., et al. 1994. Nature371, 346.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable