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Safety Information

D2650

Sigma-Aldrich

Dimethyl sulfoxide

Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Synonym(s):

DMSO

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About This Item

Linear Formula:
(CH3)2SO
CAS Number:
67-68-5
Molecular Weight:
78.13
Beilstein:
506008
EC Number:
200-664-3
MDL number:
MFCD00002089
UNSPSC Code:
12191502
PubChem Substance ID:
24893703
NACRES:
NA.75

grade

Hybri-Max™

Quality Level

400

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

sterility

sterile-filtered

product line

BioReagent

Assay

≥99.7%

form

liquid

autoignition temp.

573 °F

expl. lim.

42 %, 63 °F

technique(s)

cell culture | hybridoma: suitable
cryopreservation: suitable

impurities

endotoxin, tested
≤0.1% water
water

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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Application

DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).

Packaging

100 mL in amber bottle
Glass ampoule packaging is now available under a new product number, D2653

Other Notes

This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.
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Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

FSL

Group 4: Flammable liquids
Type 3 petroleums
Hazardous rank III
Water soluble liquid

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

D2650-1L:4548173193991
D2650-5X10ML:4548173194011
D2650-5X5ML:4548173194028
D2650-100ML:4548173193984
D2650-500ML:4548173194004
D2650-VAL:
D2650-PROC:
D2650-100ML-C:
D2650-VAR:
D2650-BULK:
D2650-5X5ML-C:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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