Genomics research has become even more fast-paced since the annotation of the human genome was completed in 2003. Sequencing technologies have evolved and expanded and are no longer only the purview of bioinformaticians. In fact, basic science researchers are now being tasked with performing and understanding next generation sequencing (NGS) techniques and workflows. In this webinar, we will explore the history of NGS with an overview of sequencing-by-synthesis chemistry, discuss applications of NGS with a spotlight on gene editing, and summarize new technologies poised to redefine the limitations of short-read NGS.
Stacey Ward, Ph.D.
Principal R&D Scientist, Cell Design Studio
Stacey received her Ph.D. in Microbiology and Immunology from SUNY Upstate Medical University in Syracuse, NY, studying the dysregulation of cell cycle proteins by varicella zoster virus. After completing a postdoc at UConn investigating herpes simplex virus DNA replication, she moved to St. Louis for a second postdoc at WashU School of Medicine dissecting the role of sonic hedgehog signaling in brain cancer. She joined MilliporeSigma in early 2016 as a member of the Cell Design Studio, a team focusing on genetically engineering cells for customers and off-the-shelf products. She helped develop the team’s current workflow using next generation sequencing for CRISPR screening and currently leads a team of scientists performing gene editing and next generation sequencing.
Senior R&D Scientist, Cell Design Studio
Jill began her career in 2007 as an Associate Scientist in the Mission Operations Functional Genomics group, where she worked with RNAi, ZFN, and CRISPR products. In 2014, she joined the Cell Design Studio, where she uses gene modifying products to engineer custom cell lines for customers. Jill received her Master's degree in Biology from Washington University in Saint Louis this past spring in 2020. In CDS, she works with a variety of cells including hard to transfect monocytes to induced pluripotent stem cell (iPSC). She was one of the leads that worked towards bringing NGS technologies in-house, and continues to support the NGS process.