The choice of a next-generation sequencing library preparation strategy depends highly on the sample origin, desired readout, and throughput. Your samples can range from infinitesimally small single cells to intact tissues and can include intact genomes or highly degraded nucleic acid fragments. The desired output of an experiment is critical: are you looking for an entire genome or a subset of targets? The number of samples to be analyzed within a single sequencing run is a third consideration when designing an experiment. During this webinar, we will discuss each of these parameters and their relationship to various library preparation methods.
Jennifer Silverman, Ph.D.
Senior Scientist, Specialty Assays R&D
Jennifer earned her Ph.D. in Microbiology from the University of Wisconsin-Madison, where she utilized both bacterial genetics and protein biochemistry to elucidate the activity of a ubiquitous protein family. She completed her postdoctoral work at Washington University in St. Louis and then took on industry roles in metabolic engineering and protein biophysics. In 2017, she joined the Specialty Assays R&D group at Merck, where she launched products including SAvPhire Monomeric Streptavidin and SEQPLEX-i WGA and WTA kits. She currently leads an R&D team developing new catalog products for NGS library preparation, molecular biology, and epigenetics.
Brian Ward, Ph.D.
Associate Fellow, Diagnostic Solutions R&D
Brian earned his Ph.D. in Physical-Organic Chemistry from Michigan State University, studying heme chemistry. He went on to become acquainted with molecular biology as a postdoctoral fellow at Syracuse University characterizing drug-DNA interactions. He joined SigmaAldrich’s first attempt at a dedicated R&D team in 1987. He has developed many products and has several patents in the area of nucleic acid chemistry.