The dysregulation of lipid metabolism is known to be associated with malignancy and metastatic progression in colorectal cancer (CRC). However, a detailed survey of the global ‘lipidomic hallmarks’ of CRC, and evaluation of whether variations in lipidome profiles between individual patient tumour samples constitute a distinct, or complementary, taxonomy relative to currently used genomic, transcriptomic or proteomic classifications, is currently lacking. Furthermore, conventional analytical strategies for lipidome analysis typically fail to provide sufficient information for precise structural characterization, and quantification, of the individual molecular lipid species that may be present within a complex sample of interest. To address these needs, I will introduce some of the key concepts of lipidomics, and then describe results from our recent efforts aimed at the development of an integrated quantitative workflow for ‘shotgun’ lipidome analysis, involving the use of stable-isotope labelled lipid standards, functional group selective derivatization and ultra-high resolution mass spectrometry and UV-photodissociation tandem mass spectrometry techniques, and the application of this workflow for the characterization of lipidome profile alterations in a series of clinical CRC tumor and normal tissue samples, and CRC cell lines.
Session 1:presented April 29, 2021
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