Ascentis^® Express RP-Amide Columns for HPLC and UHPLC

Improved separation of basic compounds

While the Ascentis^® Express C8 and C18 provide classic reversed-phase selectivity, the RP-Amide phase provides alternate reversed-phase selectivity:

• Improved peak shape for basic compounds
• 100% aqueous compatibility
• LC-MS compatibility
• Fast separation with Fused-Core^® technology

The Ascentis^® Express RP-Amide HPLC column provides increased selectivity for polar compounds, especially those that can act as a hydrogen bond donor.

Ascentis® Express RP-Amide Specifications:
Silica:	Type B (High purity silica)
Particle Platform:	Superficially porous particles (SPP)
Phase Chemistry:	C16-Amide
Particle Size:	2.0 µm / 2.7 µm / 5.0 µm
Pore Size:	90 Å
Carbon Load:	7.3% / 8.3% / 5.1%
Surface Area:	120 m²/g / 135 m²/g /90 m²/g
pH Range:	2 - 9
Max Temperature at Low pH:	60 °C
Max Temperature at High pH:	40 °C
Endcapped:	Yes

Featured Application

• Bisphenol A and Related Compounds on Ascentis Express RP-Amide

RP-Amide phase with Fused-Core^® particles for fast separation of basic compounds

Ascentis^® Express RP-Amide is a high-speed, high-performance liquid chromatography column based on a superficially porous Fused-Core^® particle design. The Fused-Core^® particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths. Three different particle sizes are available for your specific needs:

2.0 µm particles

Best Fused-Core UHPLC Column

An optimized solution for high-throughput small molecule analysis

2.7 µm particles

Fast HPLC on ANY System

A practical solution that delivers UHPLC performance from any HPLC

5.0 µm particles

The Lab Work-Horse Column

True plug and play solution for improving existing 3 or 5 µm fully porous particle HPLC columns