- Enzyme-catalyzed expressed protein ligation.
Enzyme-catalyzed expressed protein ligation.
Nature methods (2016-11-01)
Samuel H Henager, Nam Chu, Zan Chen, David Bolduc, Daniel R Dempsey, Yousang Hwang, James Wells, Philip A Cole
PMID27669326
ABSTRACT
Expressed protein ligation is a valuable method for protein semisynthesis that involves the reaction of recombinant protein C-terminal thioesters with N-terminal cysteine (N-Cys)-containing peptides, but the requirement of a Cys residue at the ligation junction can limit the utility of this method. Here we employ subtiligase variants to efficiently ligate Cys-free peptides to protein thioesters. Using this method, we have more accurately determined the effect of C-terminal phosphorylation on the tumor suppressor protein PTEN.
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Sigma-Aldrich
Anti-Biotin−Peroxidase antibody produced in goat, affinity isolated antibody, buffered aqueous solution