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Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Organic & biomolecular chemistry (2015-01-03)

Sevnur Serim, Philipp Baer, Steven H L Verhelst

ABSTRACT

Activity-based probes (ABPs) are powerful tools for the analysis of active enzyme species in whole proteomes, cells or animals. Quenched fluorescent ABPs (qABPs) can be applied for real time imaging, allowing the visualization of dynamic enzyme activation by fluorescent microscopy. Unfortunately, qABPs are only available for a few enzymes. We here describe the design and synthesis of qABPs for serine proteases based on a phosphonate ester scaffold.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

Sigma-Aldrich

Acido acetico, glacial, ACS reagent, ≥99.7%

Sigma-Aldrich

Acido acetico, glacial, ReagentPlus^®, ≥99%

Sigma-Aldrich

Solfato di magnesio, anhydrous, ReagentPlus^®, ≥99.5%

Sigma-Aldrich

2-butanone, ACS reagent, ≥99.0%

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Acido acetico, glacial, ≥99.99% trace metals basis

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Bromuro di litio, ReagentPlus^®, ≥99%

Sigma-Aldrich

Acido acetico, suitable for HPLC

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(+)- L-ascorbato di sodio, crystalline, ≥98%

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(+)- L-ascorbato di sodio, BioXtra, ≥99.0% (NT)

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Di-tert-butyl dicarbonate, ReagentPlus^®, 99%

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HATU, 97%

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(+)- L-ascorbato di sodio, powder, BioReagent, suitable for cell culture

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Solfato di magnesio, anhydrous, free-flowing, Redi-Dri^™, ReagentPlus^®, ≥99.5%

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Solfato di magnesio, BioReagent, suitable for cell culture, suitable for insect cell culture

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Bromuro di litio, anhydrous, free-flowing, Redi-Dri^™, ReagentPlus^®, ≥99%

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Copper(I) bromide, 98%

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Solfato di magnesio, puriss. p.a., drying agent, anhydrous, ≥98.0% (KT), powder (very fine)

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Acido acetico, for luminescence, BioUltra, ≥99.5% (GC)

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USP

Acido acetico, United States Pharmacopeia (USP) Reference Standard

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Acetyl chloride, reagent grade, 98%

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Solfato di magnesio, anhydrous, free-flowing, Redi-Dri^™, reagent grade, ≥97%

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Acido acetico, natural, ≥99.5%, FG

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Di-tert-butyl dicarbonate, ReagentPlus^®, ≥99%

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