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  • Isotope-based analysis of modified tRNA nucleosides correlates modification density with translational efficiency.

Isotope-based analysis of modified tRNA nucleosides correlates modification density with translational efficiency.

Angewandte Chemie (International ed. in English) (2012-10-06)
Caterina Brandmayr, Mirko Wagner, Tobias Brückl, Daniel Globisch, David Pearson, Andrea Christa Kneuttinger, Veronika Reiter, Antje Hienzsch, Susanne Koch, Ines Thoma, Peter Thumbs, Stylianos Michalakis, Markus Müller, Martin Biel, Thomas Carell
PMID23037940
ABSTRACT

Useful diversity: Quantification of modified tRNA nucleobases in different murine and porcine tissues reveals a tissue-specific overall modification content. The modification content correlates with rates of protein synthesis in vitro, suggesting a direct link between tRNA modification levels and tissue-specific translational efficiency.

MATERIALI
N° Catalogo
Marchio
Descrizione del prodotto
R4875
Sigma-Aldrich
Ribonucleasi A, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein
R6513
Sigma-Aldrich
Ribonucleasi A, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
R5125
Sigma-Aldrich
Ribonucleasi A, Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein
R4642
Sigma-Aldrich
Ribonucleasi A, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
R5503
Sigma-Aldrich
Ribonucleasi A, Type I-AS, 50-100 Kunitz units/mg protein
R5500
Sigma-Aldrich
Ribonucleasi A, Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein
R5000
Sigma-Aldrich
Ribonucleasi A, Type II-A, ≥60% (SDS-PAGE), >= 60 Kunitz units/mg protein
R7884
Sigma-Aldrich
Ribonuclease B from bovine pancreas, BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE)
R5250
Sigma-Aldrich
Ribonucleasi A, Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein
R4001
Sigma-Aldrich
Ribonuclease A-agarose, ammonium sulfate suspension, 400-1,000 units/g agarose (One ml gel will yield 12-30 units)