- Fluorescence in situ hybridisation for the identification and characterisation of prokaryotes.
Fluorescence in situ hybridisation for the identification and characterisation of prokaryotes.
Current opinion in microbiology (2003-07-02)
Michael Wagner, Matthias Horn, Holger Daims
PMID12831908
ABSTRACT
Fluorescence in situ hybridisation with rRNA-targeted nucleic acid probes can be used to directly identify microorganisms within complex samples in a few hours and therefore has widespread application in environmental and medical microbiology. The past year has seen significant methodological improvements in fluorescence in situ hybridisation, as well as in the combination of this method with other techniques for inferring functional traits of microorganisms within their environment.
MATERIALI
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Eubacteria FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH)
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Eubacteria FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH), 20 µM in water
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Escherichia coli FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH)
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Bacterial negative control FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water
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Porphyromonas gingivalis FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH)
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Akkermansia muciniphila FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water
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Proteus FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water
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Akkermansia muciniphila FISH probe-ATTO488, Probe for fluorescence in situ hybridization (FISH), 20µM in water
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Porphyromonas gingivalis FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water
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Bacterial negative control FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH),20 μM in water
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Enterococcus faecium FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH), 20µM in water
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Escherichia coli FISH probe -ATTO488, Probe for fluorescence in situ hybridization (FISH), 20µM in water
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Enterococcus faecium FISH probe – ATTO 488, Probe for fluorescence in situ hybridization (FISH), 20µM in water
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Proteus FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH),20 μM in water
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Alphaproteobacteria FISH probe-Cy3, Probe for fluorescence in situ hybridization (FISH), 20 μM in water
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Eubacteria FISH probe extension set, EUBII-Cy3 and EUBIII-Cy3 FISH (fluorescence in situ hybridization) probes for research, 20 μM in water